PMID- 20811074
OWN - NLM
STAT- MEDLINE
DCOM- 20110103
LR  - 20211020
IS  - 1535-9484 (Electronic)
IS  - 1535-9476 (Print)
IS  - 1535-9476 (Linking)
VI  - 9
IP  - 9
DP  - 2010 Sep
TI  - Quantitative proteomics and metabolomics analysis of normal human cerebrospinal 
      fluid samples.
PG  - 2063-75
LID - 10.1074/mcp.M900877-MCP200 [doi]
AB  - The analysis of cerebrospinal fluid (CSF) is used in biomarker discovery studies 
      for various neurodegenerative central nervous system (CNS) disorders. However, 
      little is known about variation of CSF proteins and metabolites between patients 
      without neurological disorders. A baseline for a large number of CSF compounds 
      appears to be lacking. To analyze the variation in CSF protein and metabolite 
      abundances in a number of well-defined individual samples of patients undergoing 
      routine, non-neurological surgical procedures, we determined the variation of 
      various proteins and metabolites by multiple analytical platforms. A total of 126 
      common proteins were assessed for biological variations between individuals by 
      ESI-Orbitrap. A large spread in inter-individual variation was observed (relative 
      standard deviations [RSDs] ranged from 18 to 148%) for proteins with both high 
      abundance and low abundance. Technical variation was between 15 and 30% for all 
      126 proteins. Metabolomics analysis was performed by means of GC-MS and nuclear 
      magnetic resonance (NMR) imaging and amino acids were specifically analyzed by 
      LC-MS/MS, resulting in the detection of more than 100 metabolites. The variation 
      in the metabolome appears to be much more limited compared with the proteome: the 
      observed RSDs ranged from 12 to 70%. Technical variation was less than 20% for 
      almost all metabolites. Consequently, an understanding of the biological 
      variation of proteins and metabolites in CSF of neurologically normal individuals 
      appears to be essential for reliable interpretation of biomarker discovery 
      studies for CNS disorders because such results may be influenced by natural 
      inter-individual variations. Therefore, proteins and metabolites with high 
      variation between individuals ought to be assessed with caution as candidate 
      biomarkers because at least part of the difference observed between the diseased 
      individuals and the controls will not be caused by the disease, but rather by the 
      natural biological variation between individuals.
FAU - Stoop, Marcel P
AU  - Stoop MP
AD  - Department of Neurology, Erasmus University Medical Centre, Rotterdam, The 
      Netherlands.
FAU - Coulier, Leon
AU  - Coulier L
FAU - Rosenling, Therese
AU  - Rosenling T
FAU - Shi, Shanna
AU  - Shi S
FAU - Smolinska, Agnieszka M
AU  - Smolinska AM
FAU - Buydens, Lutgarde
AU  - Buydens L
FAU - Ampt, Kirsten
AU  - Ampt K
FAU - Stingl, Christoph
AU  - Stingl C
FAU - Dane, Adrie
AU  - Dane A
FAU - Muilwijk, Bas
AU  - Muilwijk B
FAU - Luitwieler, Ronald L
AU  - Luitwieler RL
FAU - Sillevis Smitt, Peter A E
AU  - Sillevis Smitt PA
FAU - Hintzen, Rogier Q
AU  - Hintzen RQ
FAU - Bischoff, Rainer
AU  - Bischoff R
FAU - Wijmenga, Sybren S
AU  - Wijmenga SS
FAU - Hankemeier, Thomas
AU  - Hankemeier T
FAU - van Gool, Alain J
AU  - van Gool AJ
FAU - Luider, Theo M
AU  - Luider TM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mol Cell Proteomics
JT  - Molecular & cellular proteomics : MCP
JID - 101125647
SB  - IM
MH  - Case-Control Studies
MH  - Cerebrospinal Fluid/*metabolism
MH  - Chromatography, Liquid
MH  - Humans
MH  - Magnetic Resonance Spectroscopy
MH  - *Metabolomics
MH  - *Proteomics
MH  - Reproducibility of Results
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Tandem Mass Spectrometry
PMC - PMC2938111
EDAT- 2010/09/03 06:00
MHDA- 2011/01/05 06:00
PMCR- 2011/09/01
CRDT- 2010/09/03 06:00
PHST- 2010/09/03 06:00 [entrez]
PHST- 2010/09/03 06:00 [pubmed]
PHST- 2011/01/05 06:00 [medline]
PHST- 2011/09/01 00:00 [pmc-release]
AID - S1535-9476(20)30916-6 [pii]
AID - M110.000877 [pii]
AID - 10.1074/mcp.M900877-MCP200 [doi]
PST - ppublish
SO  - Mol Cell Proteomics. 2010 Sep;9(9):2063-75. doi: 10.1074/mcp.M900877-MCP200.