PMID- 20812951
OWN - NLM
STAT- MEDLINE
DCOM- 20110419
LR  - 20181201
IS  - 1574-695X (Electronic)
IS  - 0928-8244 (Linking)
VI  - 60
IP  - 2
DP  - 2010 Nov
TI  - Heat-killed and gamma-irradiated Brucella strain RB51 stimulates enhanced dendritic 
      cell activation, but not function compared with the virulent smooth strain 2308.
PG  - 147-55
LID - 10.1111/j.1574-695X.2010.00729.x [doi]
AB  - Brucella spp. are Gram-negative, facultative intracellular bacterial pathogens 
      that cause abortion in livestock and undulant fever in humans worldwide. Brucella 
      abortus strain 2308 is a pathogenic strain that affects cattle and humans. 
      Currently, there are no efficacious human vaccines available. However, B. abortus 
      strain RB51, which is approved by the USDA, is a live-attenuated rough vaccine 
      against bovine brucellosis. Live strain RB51 induces protection via CD4(+) and 
      CD8(+) T-cell-mediated immunity. To generate an optimal T-cell response, strong 
      innate immune responses by dendritic cells (DCs) are crucial. Because of safety 
      concerns, the use of live vaccine strain RB51 in humans is limited. Therefore, in 
      this study, we analyzed the differential ability of the same doses of live, 
      heat-killed (HK) and gamma-irradiated (IR) strain RB51 in inducing DC activation and 
      function. Smooth strain 2308, live strain RB51 and lipopolysaccharide were used 
      as controls. Studies using mouse bone marrow-derived DCs revealed that, 
      irrespective of viability, strain RB51 induced greater DC activation than smooth 
      strain 2308. Live strain RB51 induced significantly (P</=0.05) higher DC maturation 
      than HK and IR strains, and only live strain RB51-infected DCs (at multiplicity 
      of infection 1:100) induced significant (P</=0.05) tumor necrosis factor-alpha and 
      interleukin-12 secretion.
CI  - (c) 2010 Federation of European Microbiological Societies. Published by Blackwell 
      Publishing Ltd. All rights reserved.
FAU - Surendran, Naveen
AU  - Surendran N
AD  - Department of Large Animal Clinical Sciences, Center for Molecular Medicine and 
      Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, 
      Virginia Tech, Blacksburg, VA, USA.
FAU - Hiltbold, Elizabeth M
AU  - Hiltbold EM
FAU - Heid, Bettina
AU  - Heid B
FAU - Sriranganathan, Nammalwar
AU  - Sriranganathan N
FAU - Boyle, Stephen M
AU  - Boyle SM
FAU - Zimmerman, Kurt L
AU  - Zimmerman KL
FAU - Witonsky, Sharon G
AU  - Witonsky SG
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20100831
PL  - England
TA  - FEMS Immunol Med Microbiol
JT  - FEMS immunology and medical microbiology
JID - 9315554
RN  - 0 (B7-2 Antigen)
RN  - 0 (Brucella Vaccine)
RN  - 0 (CD40 Antigens)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Vaccines, Inactivated)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Animals
MH  - B7-2 Antigen/analysis
MH  - Bacterial Typing Techniques
MH  - Brucella Vaccine/*immunology
MH  - Brucella abortus/*immunology/pathogenicity/radiation effects
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD40 Antigens/analysis
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Dendritic Cells/*immunology/metabolism
MH  - Flow Cytometry
MH  - Gamma Rays
MH  - Hot Temperature
MH  - Immunity, Cellular
MH  - Interleukin-12/metabolism
MH  - Lipopolysaccharides
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Vaccines, Attenuated/immunology
MH  - Vaccines, Inactivated/immunology
EDAT- 2010/09/04 06:00
MHDA- 2011/04/20 06:00
CRDT- 2010/09/04 06:00
PHST- 2010/09/04 06:00 [entrez]
PHST- 2010/09/04 06:00 [pubmed]
PHST- 2011/04/20 06:00 [medline]
AID - FIM729 [pii]
AID - 10.1111/j.1574-695X.2010.00729.x [doi]
PST - ppublish
SO  - FEMS Immunol Med Microbiol. 2010 Nov;60(2):147-55. doi: 
      10.1111/j.1574-695X.2010.00729.x. Epub 2010 Aug 31.