PMID- 20813133
OWN - NLM
STAT- MEDLINE
DCOM- 20110517
LR  - 20191210
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 170
IP  - 1-2
DP  - 2010 Dec
TI  - Development of a loop-mediated isothermal amplification assay for rapid and 
      sensitive detection of ostreid herpesvirus 1 DNA.
PG  - 30-6
LID - 10.1016/j.jviromet.2010.08.015 [doi]
AB  - A loop-mediated isothermal amplification (LAMP) assay was developed for rapid, 
      specific and sensitive detection of ostreid herpesvirus 1 (OsHV-1) DNA. A set of 
      four primers was designed, based on the sequence of the ATPase subunit of the 
      OsHV-1 DNA-packaging terminase gene. The reaction temperature and time were 
      optimized to 64 degrees C and 60min, respectively. LAMP products were detected by agarose 
      gel electrophoresis or by visual inspection of a color change due to addition of 
      fluorescent dye. The developed method was highly specific for detection of 
      OsHV-1, and no cross-reaction was observed with other DNA viruses, such as White 
      spot syndrome virus (WSSV), Penaeus stylirostris densovirus (PstDNV), Turbot 
      reddish body iridovirus (TRBIV) and Lymphocystis disease virus (LCDV) found 
      commonly in China. The lower detection limit of the LAMP assay was approximately 
      20 copies per reaction, and it was 100 times more sensitive than that of 
      conventional PCR. A comparative evaluation of 10 oyster samples using LAMP and 
      PCR assays showed overall correlation in positive and negative results for 
      OsHV-1. These results indicate that the LAMP assay is a simple, rapid, sensitive, 
      specific and reliable technique for the detection of OsHV-1. The LAMP technique 
      has capacity for use for the detection of OsHV-1 both in the laboratory and on 
      farms.
CI  - Crown Copyright (c) 2010. Published by Elsevier B.V. All rights reserved.
FAU - Ren, Weicheng
AU  - Ren W
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science, 106 
      Nanjing Road, Qingdao, Shandong, China.
FAU - Renault, Tristan
AU  - Renault T
FAU - Cai, Yuyong
AU  - Cai Y
FAU - Wang, Chongming
AU  - Wang C
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100908
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Crassostrea/*virology
MH  - DNA Primers
MH  - DNA Viruses/classification/genetics/*isolation & purification
MH  - DNA, Viral/analysis/*isolation & purification
MH  - Electrophoresis, Agar Gel
MH  - Fluorescent Dyes
MH  - Nucleic Acid Amplification Techniques/*methods
MH  - Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - Staining and Labeling/methods
EDAT- 2010/09/04 06:00
MHDA- 2011/05/18 06:00
CRDT- 2010/09/04 06:00
PHST- 2010/03/09 00:00 [received]
PHST- 2010/08/17 00:00 [revised]
PHST- 2010/08/23 00:00 [accepted]
PHST- 2010/09/04 06:00 [entrez]
PHST- 2010/09/04 06:00 [pubmed]
PHST- 2011/05/18 06:00 [medline]
AID - S0166-0934(10)00307-1 [pii]
AID - 10.1016/j.jviromet.2010.08.015 [doi]
PST - ppublish
SO  - J Virol Methods. 2010 Dec;170(1-2):30-6. doi: 10.1016/j.jviromet.2010.08.015. 
      Epub 2010 Sep 8.