PMID- 20814816
OWN - NLM
STAT- MEDLINE
DCOM- 20120913
LR  - 20220321
IS  - 1573-7217 (Electronic)
IS  - 0167-6806 (Linking)
VI  - 128
IP  - 3
DP  - 2011 Aug
TI  - Clinical significance of high focal adhesion kinase gene copy number and 
      overexpression in invasive breast cancer.
PG  - 647-55
LID - 10.1007/s10549-010-1150-2 [doi]
AB  - Increased expression and activation of focal adhesion kinase (FAK) was reported 
      to be an unfavorable factor in various human cancers, including breast cancer. In 
      order to study FAK gene status as a prognostic factor, we evaluated FAK gene copy 
      number and FAK protein expression in invasive breast cancer. Tumors from 435 
      patients with invasive breast cancer were evaluated for FAK gene status using 
      fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) based on 
      tissue array method. Survival analyses were performed using the Kaplan-Meier 
      method in 267 patients. 42 out of 362 evaluable cases (11.6%) showed high 
      polysomy and 22 cases (6.1%) had gene amplification by FISH. 108 out of 393 
      evaluable cases (27.5%) showed FAK overexpression by IHC. FAK FISH positivity was 
      significantly associated with higher histologic grade, higher T stage, negative 
      estrogen receptor expression, negative progesterone receptor expression and 
      triple-negative phenotype; FAK overexpression with higher histologic grade and 
      triple-negative phenotype. FAK overexpression was noted in 57.8% (37 of 64) of 
      FAK FISH+ cases. The concordance of FISH and IHC results for FAK gene was 
      observed in 74.9% (271 of 362). Survival analyses revealed the patients with FAK 
      FISH+ breast cancer had significantly shorter overall survival and relapse-free 
      survival compared to those with FISH- breast cancer. In node-positive breast 
      cancer patients who received postoperative systemic treatment, the patients with 
      FAK FISH+ showed significantly shorter 5-year survival rates. Despite of high 
      significant concordance between the results of FISH and IHC for FAK gene in 
      invasive breast cancer, only FAK FISH positivity was an unfavorable prognostic 
      factor.
FAU - Yom, Cha Kyong
AU  - Yom CK
AD  - Department of Surgery, Cancer Research Institute, Seoul National University 
      College of Medicine, Seoul, Korea.
FAU - Noh, Dong-Young
AU  - Noh DY
FAU - Kim, Woo Ho
AU  - Kim WH
FAU - Kim, Hee Sung
AU  - Kim HS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100903
PL  - Netherlands
TA  - Breast Cancer Res Treat
JT  - Breast cancer research and treatment
JID - 8111104
RN  - EC 2.7.10.2 (Focal Adhesion Protein-Tyrosine Kinases)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Breast Neoplasms/diagnosis/*enzymology/*genetics/mortality/pathology
MH  - DNA Copy Number Variations/*genetics
MH  - Female
MH  - Focal Adhesion Protein-Tyrosine Kinases/*genetics/metabolism
MH  - Gene Expression/*genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Prognosis
MH  - Survival Analysis
EDAT- 2010/09/04 06:00
MHDA- 2012/09/14 06:00
CRDT- 2010/09/04 06:00
PHST- 2010/06/25 00:00 [received]
PHST- 2010/08/23 00:00 [accepted]
PHST- 2010/09/04 06:00 [entrez]
PHST- 2010/09/04 06:00 [pubmed]
PHST- 2012/09/14 06:00 [medline]
AID - 10.1007/s10549-010-1150-2 [doi]
PST - ppublish
SO  - Breast Cancer Res Treat. 2011 Aug;128(3):647-55. doi: 10.1007/s10549-010-1150-2. 
      Epub 2010 Sep 3.