PMID- 20816080
OWN - NLM
STAT- MEDLINE
DCOM- 20101217
LR  - 20211020
IS  - 1542-0086 (Electronic)
IS  - 0006-3495 (Print)
IS  - 0006-3495 (Linking)
VI  - 99
IP  - 5
DP  - 2010 Sep 8
TI  - Detecting protein aggregates on untreated human tissue samples by atomic force 
      microscopy recognition imaging.
PG  - 1660-7
LID - 10.1016/j.bpj.2010.06.044 [doi]
AB  - We apply topography and recognition (TREC) imaging to the analysis of whole, 
      untreated human tissue for what we believe to be the first time. 
      Pseudoexfoliation syndrome (PEX), a well-known cause of irreversible blindness 
      worldwide, is characterized by abnormal protein aggregation on the anterior lens 
      capsule of the eye. However, the development of effective therapies has been 
      hampered by a lack of detailed knowledge of the protein constituents in these 
      pathological deposits and their distribution. Using both TREC and 
      immunofluorescence, one of the proteins implicated in the PEX pathology--the 
      apolipoprotein clusterin--was detected, and differences in its distribution 
      pattern on the surface of untreated human lens capsule tissue in both PEX and 
      normal control samples were investigated. Our study shows the potential of TREC 
      imaging for the analysis of whole, untreated human tissue samples.
CI  - Copyright 2010 Biophysical Society. Published by Elsevier Inc. All rights 
      reserved.
FAU - Creasey, Rhiannon
AU  - Creasey R
AD  - School of Chemical and Physical Sciences, Flinders University, Bedford Park, 
      Australia.
FAU - Sharma, Shiwani
AU  - Sharma S
FAU - Craig, Jamie E
AU  - Craig JE
FAU - Gibson, Christopher T
AU  - Gibson CT
FAU - Ebner, Andreas
AU  - Ebner A
FAU - Hinterdorfer, Peter
AU  - Hinterdorfer P
FAU - Voelcker, Nicolas H
AU  - Voelcker NH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biophys J
JT  - Biophysical journal
JID - 0370626
RN  - 0 (Antibodies)
RN  - 0 (Clusterin)
SB  - IM
MH  - Antibodies/immunology
MH  - Clusterin/*chemistry/immunology/*metabolism
MH  - Exfoliation Syndrome/diagnosis/metabolism/pathology
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Lens Capsule, Crystalline/metabolism
MH  - Microscopy, Atomic Force/*methods
MH  - Molecular Imaging/*methods
MH  - *Protein Multimerization
MH  - Protein Structure, Quaternary
PMC - PMC2931734
EDAT- 2010/09/08 06:00
MHDA- 2010/12/18 06:00
PMCR- 2011/09/08
CRDT- 2010/09/07 06:00
PHST- 2010/02/13 00:00 [received]
PHST- 2010/06/15 00:00 [revised]
PHST- 2010/06/18 00:00 [accepted]
PHST- 2010/09/07 06:00 [entrez]
PHST- 2010/09/08 06:00 [pubmed]
PHST- 2010/12/18 06:00 [medline]
PHST- 2011/09/08 00:00 [pmc-release]
AID - S0006-3495(10)00787-3 [pii]
AID - BPJ1931 [pii]
AID - 10.1016/j.bpj.2010.06.044 [doi]
PST - ppublish
SO  - Biophys J. 2010 Sep 8;99(5):1660-7. doi: 10.1016/j.bpj.2010.06.044.