PMID- 20816209
OWN - NLM
STAT- MEDLINE
DCOM- 20101214
LR  - 20100906
IS  - 1557-7988 (Electronic)
IS  - 0076-6879 (Linking)
VI  - 480
DP  - 2010
TI  - Carbohydrate signaling by C-type lectin DC-SIGN affects NF-kappaB activity.
PG  - 151-64
LID - 10.1016/S0076-6879(10)80008-4 [doi]
AB  - Pathogen recognition is central to the induction of adaptive immunity. Dendritic 
      cells (DCs) express different pattern recognition receptors (PRRs), such as 
      Toll-like receptors and C-type lectins, that sense invading pathogens. Pathogens 
      trigger a specific set of PRRs, leading to activation of intracellular signaling 
      processes that shapes the adaptive immunity. It is becoming clear that cross talk 
      between these signaling routes is crucial for pathogen-tailored immune responses. 
      The C-type lectin DC-SIGN interacts with different mannose-expressing pathogens 
      such as Mycobacterium tuberculosis and HIV-1. Notably, DC-SIGN triggering by 
      these pathogens results in a specific Raf-1-dependent signaling pathway that 
      modulates TLR-induced NF-kappaB activation. Here, we will discuss the various 
      methods that we have used to identify the innate signaling by the C-type lectin 
      DC-SIGN, and how to analyze the consequences on NF-kappaB activation.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Gringhuis, Sonja I
AU  - Gringhuis SI
AD  - Center of Infection and Immunity Amsterdam, Academic Medical Center, University 
      of Amsterdam, Amsterdam, The Netherlands.
FAU - Geijtenbeek, Teunis B H
AU  - Geijtenbeek TB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - United States
TA  - Methods Enzymol
JT  - Methods in enzymology
JID - 0212271
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Receptors, Cell Surface)
RN  - EC 2.3.1.- (Acetyltransferases)
SB  - IM
MH  - Acetyltransferases/metabolism/physiology
MH  - Animals
MH  - Carbohydrate Metabolism/*physiology
MH  - Cell Adhesion Molecules/chemistry/metabolism/*physiology
MH  - Dendritic Cells/drug effects/immunology/metabolism
MH  - Gene Expression Regulation/physiology
MH  - Humans
MH  - Lectins, C-Type/chemistry/metabolism/*physiology
MH  - Lipopolysaccharides/pharmacology
MH  - NF-kappa B/*metabolism/physiology
MH  - Protein Processing, Post-Translational
MH  - RNA Interference/physiology
MH  - Receptors, Cell Surface/chemistry/metabolism/*physiology
MH  - Signal Transduction/drug effects/physiology
EDAT- 2010/09/08 06:00
MHDA- 2010/12/16 06:00
CRDT- 2010/09/07 06:00
PHST- 2010/09/07 06:00 [entrez]
PHST- 2010/09/08 06:00 [pubmed]
PHST- 2010/12/16 06:00 [medline]
AID - S0076-6879(10)80008-4 [pii]
AID - 10.1016/S0076-6879(10)80008-4 [doi]
PST - ppublish
SO  - Methods Enzymol. 2010;480:151-64. doi: 10.1016/S0076-6879(10)80008-4.