PMID- 20816936
OWN - NLM
STAT- MEDLINE
DCOM- 20101130
LR  - 20181201
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 401
IP  - 1
DP  - 2010 Oct 8
TI  - Bone morphogenetic protein 6 drives both osteogenesis and chondrogenesis in 
      murine adipose-derived mesenchymal cells depending on culture conditions.
PG  - 20-5
LID - 10.1016/j.bbrc.2010.08.135 [doi]
AB  - Bone morphogenetic proteins (BMPs) play a dual role as a factor in both bone and 
      cartilage development and correspondingly have the therapeutic potential to 
      regenerate both tissues. Given this dual nature, previous in vitro research using 
      BMPs has relied on distinct media formulations and culture conditions to drive 
      undifferentiated cells to the osteogenic or chondrogenic lineage. To isolate the 
      impact of culture conditions and to explore the effect of BMP-6 on murine 
      adipose-derived mesenchymal cells (ASCs), ASCs were seeded in either monolayer or 
      pellets in an identical medium containing BMP-6. Results indicate that BMP-6 
      differentially promotes osteogenesis and chondrogenesis in ASCs depending on 
      culture conditions. BMP-6 potently induced alkaline phosphatase activity and 
      mineralization in ASCs cultured in monolayer conditions. In contrast, BMP-6 
      enhanced proteoglycan accumulation in ASCs seeded in chondrogenic pellet culture. 
      A comparison of gene expression suggests that the differentiating effect of BMP-6 
      is specific to the particular culture condition. This study highlights the 
      importance of the interactions between chemical signaling and microenvironmental 
      cues in directing cell fate.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Kemmis, Carly M
AU  - Kemmis CM
AD  - Department of Mechanical Engineering and Bioengineering Graduate Program, 
      University of Notre Dame, Notre Dame, IN 46556, USA. ckemmis@gmail.com
FAU - Vahdati, Ali
AU  - Vahdati A
FAU - Weiss, Holly E
AU  - Weiss HE
FAU - Wagner, Diane R
AU  - Wagner DR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20100909
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Bone Morphogenetic Protein 6)
RN  - 0 (Culture Media)
SB  - IM
MH  - Adipose Tissue/*cytology
MH  - Animals
MH  - Bone Morphogenetic Protein 6/*pharmacology/physiology
MH  - *Cell Culture Techniques
MH  - Cells, Cultured
MH  - *Chondrogenesis
MH  - Culture Media/chemistry/pharmacology
MH  - Male
MH  - Mesenchymal Stem Cells/*drug effects/physiology
MH  - Mice
MH  - Mice, Inbred Strains
MH  - *Osteogenesis
EDAT- 2010/09/08 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/09/07 06:00
PHST- 2010/08/19 00:00 [received]
PHST- 2010/08/29 00:00 [accepted]
PHST- 2010/09/07 06:00 [entrez]
PHST- 2010/09/08 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - S0006-291X(10)01656-6 [pii]
AID - 10.1016/j.bbrc.2010.08.135 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2010 Oct 8;401(1):20-5. doi: 
      10.1016/j.bbrc.2010.08.135. Epub 2010 Sep 9.