PMID- 20817447
OWN - NLM
STAT- MEDLINE
DCOM- 20110110
LR  - 20131121
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 54
IP  - 1
DP  - 2011 Jan 5
TI  - Optimization and validation of the micellar electrokinetic capillary 
      chromatographic method for simultaneous determination of sulfonamide and 
      amphenicol-type drugs in poultry tissue.
PG  - 160-7
LID - 10.1016/j.jpba.2010.08.005 [doi]
AB  - The report describes a new approach enabling simple and rapid multi-residue 
      screening of seven sulfonamides (SAs): sulfamethazine (SMZ), sulfamerazine (SMR), 
      sulfathiazole (STZ), sulfachloropyridazine (SCP), sulfamethoxazole (SMX), 
      sulfacarbamide (SC), and sulfaguanidine (SG); and three amphenicol-type 
      antibiotics: chloramphenicol (CAP), thiamphenicol (TAP), and florfenicol (FF) in 
      animal tissue by micellar electrokinetic chromatography (MEKC). The analytes were 
      isolated from tissue samples through solid-phase extraction (with a C(18) 
      cartridges) following protein precipitation with acetonitrile. The evaluated LOD 
      and LOQ values ranged from 1.3 to 7.8 and from 4.5 to 26.1 ng/g, respectively. 
      These values are far lower than the maximum residue limits (MRLs) set by several 
      control authorities. Intra- and inter-day precision data were less than 9.5% and 
      11.2% for SAs, and 8.4% and 14.9% for amphenicols. Moreover, the method was found 
      accurate, with the recoveries ranging from 86.4% to 109.4%. The absolute 
      recoveries of the analysed drugs were higher than 77.2%. The results obtained in 
      the validation process demonstrate that the developed CE method is suitable for 
      simultaneous determination of SA and amphenicol residues in poultry tissue, with 
      the total run time less than 8 min.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Kowalski, Piotr
AU  - Kowalski P
AD  - Medical University of Gdansk, Faculty of Pharmacy, Department of Pharmaceutical 
      Chemistry, Hallera 107, PL 80-416 Gdansk, Poland. piotrpl@wp.pl
FAU - Plenis, Alina
AU  - Plenis A
FAU - Oledzka, Ilona
AU  - Oledzka I
FAU - Konieczna, Lucyna
AU  - Konieczna L
LA  - eng
PT  - Journal Article
DEP - 20100817
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Acetonitriles)
RN  - 0 (Sulfonamides)
RN  - FLQ7571NPM (Thiamphenicol)
RN  - Z072SB282N (acetonitrile)
SB  - IM
MH  - Acetonitriles/chemistry
MH  - Animals
MH  - Calibration
MH  - *Chemistry Techniques, Analytical
MH  - Chemistry, Pharmaceutical/methods
MH  - Chromatography, Micellar Electrokinetic Capillary/*methods
MH  - Electrophoresis, Capillary/methods
MH  - Models, Chemical
MH  - Muscles/metabolism
MH  - Poultry
MH  - Reproducibility of Results
MH  - Sulfonamides/*chemistry
MH  - Thiamphenicol/*chemistry
MH  - Time Factors
EDAT- 2010/09/08 06:00
MHDA- 2011/01/11 06:00
CRDT- 2010/09/07 06:00
PHST- 2010/02/10 00:00 [received]
PHST- 2010/07/28 00:00 [revised]
PHST- 2010/08/08 00:00 [accepted]
PHST- 2010/09/07 06:00 [entrez]
PHST- 2010/09/08 06:00 [pubmed]
PHST- 2011/01/11 06:00 [medline]
AID - S0731-7085(10)00465-6 [pii]
AID - 10.1016/j.jpba.2010.08.005 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2011 Jan 5;54(1):160-7. doi: 10.1016/j.jpba.2010.08.005. 
      Epub 2010 Aug 17.