PMID- 20819436
OWN - NLM
STAT- MEDLINE
DCOM- 20100928
LR  - 20170214
IS  - 0300-0605 (Print)
IS  - 0300-0605 (Linking)
VI  - 38
IP  - 3
DP  - 2010 May-Jun
TI  - Growth suppression of human laryngeal squamous cell carcinoma by 
      adenoviral-mediated interleukin-12.
PG  - 994-1004
AB  - This study explored the inhibitory role of the adenoviral-mediated-interleukin 
      (IL)-12 (Ad.mIL-12) gene in the growth of laryngeal squamous cell carcinoma 
      (LSCC). Human epithelial type 2 (Hep-2) cells were transfected with Ad.mIL-12, 
      and IL-12 gene expression of the cells was evaluated. The proliferation and 
      apoptosis of Hep-2 cells in vitro were detected by MTT 
      (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and flow 
      cytometry. Experimental tumours in mice were injected intratumourally with the 
      same recombinant adenoviruses and inhibition of tumour growth observed. Apoptosis 
      in Hep-2 xenotransplants was detected using TUNEL (terminal deoxynucleotidyl 
      transferase dUTP nick-end labelling) assay and transmission electron microscopy. 
      The expression of IL-12 in Ad.mIL-12 transfected Hep-2 cells was significantly 
      increased. In vitro, Ad.mIL-12 decreased the viability of and increased apoptosis 
      in Hep-2 cells. Increased apoptosis was also seen in vivo. The mean weight and 
      volume of tumours in Ad.mIL-12 treated mice were significantly lower than in the 
      control group. It is concluded that Ad.mIL-12 can suppress LSCC growth and induce 
      apoptosis.
FAU - Tian, L
AU  - Tian L
AD  - Department of Otorhinolaryngology/Head and Neck Surgery, The Second Affiliated 
      Hospital of Harbin Medical University, Harbin, China.
FAU - Chen, X
AU  - Chen X
FAU - Sun, Y
AU  - Sun Y
FAU - Liu, M
AU  - Liu M
FAU - Zhu, D
AU  - Zhu D
FAU - Ren, J
AU  - Ren J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Int Med Res
JT  - The Journal of international medical research
JID - 0346411
RN  - 0 (Tetrazolium Salts)
RN  - 0 (Thiazoles)
RN  - 187348-17-0 (Interleukin-12)
RN  - EUY85H477I (thiazolyl blue)
SB  - IM
MH  - Adenoviridae/*genetics
MH  - Animals
MH  - Apoptosis
MH  - Carcinoma, Squamous Cell/metabolism/*pathology/therapy
MH  - Cell Survival
MH  - Genetic Therapy/*methods
MH  - *Genetic Vectors
MH  - Hep G2 Cells/ultrastructure/virology
MH  - Humans
MH  - Interleukin-12/genetics/metabolism
MH  - Laryngeal Neoplasms/metabolism/*pathology/therapy
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Microscopy, Electron, Transmission
MH  - Tetrazolium Salts/metabolism
MH  - Thiazoles/metabolism
MH  - Transfection
MH  - Xenograft Model Antitumor Assays
EDAT- 2010/09/08 06:00
MHDA- 2010/09/30 06:00
CRDT- 2010/09/08 06:00
PHST- 2010/09/08 06:00 [entrez]
PHST- 2010/09/08 06:00 [pubmed]
PHST- 2010/09/30 06:00 [medline]
AID - 10.1177/147323001003800326 [doi]
PST - ppublish
SO  - J Int Med Res. 2010 May-Jun;38(3):994-1004. doi: 10.1177/147323001003800326.