PMID- 20819641
OWN - NLM
STAT- MEDLINE
DCOM- 20101223
LR  - 20181201
IS  - 2542-5641 (Electronic)
IS  - 0366-6999 (Linking)
VI  - 123
IP  - 13
DP  - 2010 Jul
TI  - Calcitonin gene-related peptide induces proliferation and monocyte 
      chemoattractant protein-1 expression via extracellular signal-regulated kinase 
      activation in rat osteoblasts.
PG  - 1748-53
AB  - BACKGROUND: Calcitonin gene-related peptide (CGRP), a sensory neuropeptide, 
      affects osteoblast proliferation and bone formation. However, the mechanisms are 
      not fully understood. Monocyte chemoattractant protein-1 (MCP-1) is a chemokine 
      that stimulates the migration of monocytes and plays important roles in 
      regulating bone remolding during fracture repair. In this study, we investigated 
      the effects of CGRP on proliferation and MCP-1 expression in cultured rat 
      osteoblasts. METHODS: Primary rat osteoblasts were isolated from fetal rats 
      calvariae. Cells were exposed to gradient concentrations (10(-9) to 10(-7) mol/L) 
      of CGRP. Protein and mRNA levels of MCP-1 were quantified by Western blotting and 
      semiquantitative reverse transcription-polymerase chain reaction, respectively. 
      The protein level of MCP-1 was investigated and compared in cell culture media by 
      enzyme linked immunosorbent assay (ELISA). Phospho-extracellular signal-regulated 
      kinase (ERK) expression was detected by Western blotting. Cell proliferative 
      activity was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium 
      bromide (MTT) and BrdU assay. The effects of MAPK/ERK kinase (MEK)-inhibitor 
      U0126 on CGRP-induced MCP-1 expression in primary rat osteoblasts were examined. 
      RESULTS: CGRP effectively enhanced primary rat osteoblast proliferation and led 
      to significant increases in the expression of MCP-1 mRNA and protein in time- and 
      dose-dependent manners. CGRP activated the ERK pathway. Pretreatment of cultured 
      rat osteoblasts with MEK inhibitor U0126 resulted in dose-dependent inhibitions 
      of CGRP-induced MCP-1 mRNA and protein levels. Thus, CGRP promoted cell 
      proliferation and stimulated MCP-1 expression in cultured rat osteoblasts. 
      CONCLUSION: These studies document novel links between CGRP and MCP-1 and 
      illuminate the effects of CGRP in regulating bone remodeling.
FAU - Han, Na
AU  - Han N
AD  - Department of Orthopaedics and Trauma, Peking University People's Hospital, 
      Beijing 100044, China.
FAU - Zhang, Dian-Ying
AU  - Zhang DY
FAU - Wang, Tian-Bing
AU  - Wang TB
FAU - Zhang, Pei-Xun
AU  - Zhang PX
FAU - Jiang, Bao-Guo
AU  - Jiang BG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (Butadienes)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Nitriles)
RN  - 0 (U 0126)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - JHB2QIZ69Z (Calcitonin Gene-Related Peptide)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Butadienes/pharmacology
MH  - Calcitonin Gene-Related Peptide/*pharmacology
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors/*metabolism
MH  - Nitriles/pharmacology
MH  - Osteoblasts/*drug effects/*metabolism
MH  - Rats
EDAT- 2010/09/08 06:00
MHDA- 2010/12/25 06:00
CRDT- 2010/09/08 06:00
PHST- 2010/09/08 06:00 [entrez]
PHST- 2010/09/08 06:00 [pubmed]
PHST- 2010/12/25 06:00 [medline]
PST - ppublish
SO  - Chin Med J (Engl). 2010 Jul;123(13):1748-53.