PMID- 20821059
OWN - NLM
STAT- MEDLINE
DCOM- 20110218
LR  - 20240204
IS  - 0219-1032 (Electronic)
IS  - 1016-8478 (Linking)
VI  - 30
IP  - 4
DP  - 2010 Oct
TI  - Reproductive fitness and dietary choice behavior of the genetic model organism 
      Caenorhabditis elegans under semi-natural conditions.
PG  - 347-53
LID - 10.1007/s10059-010-0125-9 [doi]
AB  - Laboratory breeding conditions of the model organism C. elegans do not correspond 
      with the conditions in its natural soil habitat. To assess the consequences of 
      the differences in environmental conditions, the effects of air composition, 
      medium and bacterial food on reproductive fitness and/or dietary-choice behavior 
      of C. elegans were investigated. The reproductive fitness of C. elegans was 
      maximal under oxygen deficiency and not influenced by a high fractional share of 
      carbon dioxide. In media approximating natural soil structure, reproductive 
      fitness was much lower than in standard laboratory media. In seminatural media, 
      the reproductive fitness of C. elegans was low with the standard laboratory food 
      bacterium E. coli (gamma-Proteobacteria), but significantly higher with C. 
      arvensicola (Bacteroidetes) and B. tropica (beta-Proteobacteria) as food. 
      Dietary-choice experiments in semi-natural media revealed a low preference of C. 
      elegans for E. coli but significantly higher preferences for C. arvensicola and 
      B. tropica (among other bacteria). Dietary-choice experiments under quasi-natural 
      conditions, which were feasible by fluorescence in situ hybridization (FISH) of 
      bacteria, showed a high preference of C. elegans for 
      Cytophaga-Flexibacter-Bacteroides, Firmicutes, and beta-Proteobacteria, but a low 
      preference for gamma-Proteobacteria. The results show that data on C. elegans under 
      standard laboratory conditions have to be carefully interpreted with respect to 
      their biological significance.
FAU - Freyth, Katharina
AU  - Freyth K
AD  - Institute of Zoophysiology, University of Muenster, Muenster, Germany.
FAU - Janowitz, Tim
AU  - Janowitz T
FAU - Nunes, Frank
AU  - Nunes F
FAU - Voss, Melanie
AU  - Voss M
FAU - Heinick, Alexander
AU  - Heinick A
FAU - Bertaux, Joanne
AU  - Bertaux J
FAU - Scheu, Stefan
AU  - Scheu S
FAU - Paul, Rudiger J
AU  - Paul RJ
LA  - eng
PT  - Journal Article
DEP - 20100902
PL  - United States
TA  - Mol Cells
JT  - Molecules and cells
JID - 9610936
RN  - 0 (Soil)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Air Pressure
MH  - Animals
MH  - Bacteroides
MH  - Behavior, Animal/physiology
MH  - Betaproteobacteria
MH  - Caenorhabditis elegans/microbiology/*physiology
MH  - Cytophaga
MH  - Ecosystem
MH  - Flexibacter
MH  - Food
MH  - Gammaproteobacteria
MH  - *Genetic Fitness/physiology
MH  - In Situ Hybridization, Fluorescence
MH  - Oxygen/analysis
MH  - Soil
MH  - Soil Microbiology
EDAT- 2010/09/08 06:00
MHDA- 2011/02/22 06:00
CRDT- 2010/09/08 06:00
PHST- 2010/04/07 00:00 [received]
PHST- 2010/06/24 00:00 [accepted]
PHST- 2010/06/22 00:00 [revised]
PHST- 2010/09/08 06:00 [entrez]
PHST- 2010/09/08 06:00 [pubmed]
PHST- 2011/02/22 06:00 [medline]
AID - S1016-8478(23)11111-3 [pii]
AID - 10.1007/s10059-010-0125-9 [doi]
PST - ppublish
SO  - Mol Cells. 2010 Oct;30(4):347-53. doi: 10.1007/s10059-010-0125-9. Epub 2010 Sep 
      2.