PMID- 20821296
OWN - NLM
STAT- MEDLINE
DCOM- 20101223
LR  - 20100907
IS  - 1869-1889 (Electronic)
IS  - 1674-7305 (Linking)
VI  - 53
IP  - 8
DP  - 2010 Aug
TI  - Quantitative assessment of toxic and nontoxic Microcystis colonies in natural 
      environments using fluorescence in situ hybridization and flow cytometry.
PG  - 973-80
LID - 10.1007/s11427-010-4038-9 [doi]
AB  - Toxic cyanobacterial blooms constitute a threat to human safety because 
      Microcystis sp. releases microcystins during growth, and particularly during cell 
      death. Therefore, analysis of toxic and nontoxic Microcystis in natural 
      communities is required in order to assess and predict bloom dynamics and toxin 
      production by these organisms. In this study, an analysis combining fluorescence 
      in situ hybridization (FISH) with flow cytometry (FCM) was used to discriminate 
      between toxic and nontoxic Microcystis and also to quantify the percentage of 
      toxic Microcystis present in blooms. The results demonstrate that the combination 
      of FISH and flow cytometry is a useful approach for studying the ecology of 
      Microcystis toxin production and for providing an early warning for toxic 
      Microcystis blooms.
FAU - Gan, NanQin
AU  - Gan N
AD  - State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of 
      Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
FAU - Huang, Qun
AU  - Huang Q
FAU - Zheng, LingLing
AU  - Zheng L
FAU - Song, LiRong
AU  - Song L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100907
PL  - China
TA  - Sci China Life Sci
JT  - Science China. Life sciences
JID - 101529880
RN  - 0 (DNA Primers)
SB  - IM
MH  - Base Sequence
MH  - DNA Primers
MH  - Flow Cytometry
MH  - In Situ Hybridization, Fluorescence
MH  - Microcystis/*isolation & purification
MH  - Polymerase Chain Reaction
EDAT- 2010/09/08 06:00
MHDA- 2010/12/25 06:00
CRDT- 2010/09/08 06:00
PHST- 2009/10/10 00:00 [received]
PHST- 2009/11/17 00:00 [accepted]
PHST- 2010/09/08 06:00 [entrez]
PHST- 2010/09/08 06:00 [pubmed]
PHST- 2010/12/25 06:00 [medline]
AID - 10.1007/s11427-010-4038-9 [doi]
PST - ppublish
SO  - Sci China Life Sci. 2010 Aug;53(8):973-80. doi: 10.1007/s11427-010-4038-9. Epub 
      2010 Sep 7.