PMID- 20822320
OWN - NLM
STAT- MEDLINE
DCOM- 20101116
LR  - 20220311
IS  - 0261-1929 (Print)
IS  - 0261-1929 (Linking)
VI  - 38
IP  - 4
DP  - 2010 Aug
TI  - A comparative evaluation of in vitro skin sensitisation tests: the human 
      cell-line activation test (h-CLAT) versus the local lymph node assay (LLNA).
PG  - 275-84
AB  - We previously developed the human cell-line activation test (h-CLAT) in vitro 
      skin sensitisation test, based on our reported finding that a 24-hour exposure of 
      THP-1 cells (a human monocytic leukaemia cell line) to sensitisers is sufficient 
      to induce the augmented expression of CD86 and CD54. The aim of this study is to 
      confirm the predictive value of h-CLAT for skin sensitisation activity by 
      employing a larger number of test chemicals. One hundred chemicals were selected, 
      according to their categorisation in the local lymph node assay (LLNA), as being: 
      extreme, strong, moderate and weak sensitisers, and non-sensitisers. The 
      correlation of the h-CLAT results with the LLNA results was 84%. There were some 
      false negatives (e.g. benzoyl peroxide, hexyl cinnamic aldehyde) and some false 
      positives (e.g. 1-bromobutane, diethylphthalate). Eight out of the 9 false 
      negatives (89%) were water-insoluble chemicals. The h-CLAT could positively 
      predict not only extreme and strong sensitisers, but also moderate and weak 
      sensitisers, though the detection rates of weak sensitisers and non-sensitisers 
      were comparatively low. Some sensitisers enhanced both CD86 and CD54 levels, and 
      some enhanced the level of only one of them. The use of the combination of CD86 
      and CD54 induction as a positive indicator, improved the accuracy of the test. In 
      conclusion, the h-CLAT is expected to be a useful cell-based in vitro method for 
      predicting skin sensitisation potential.
CI  - 2010 FRAME.
FAU - Ashikaga, Takao
AU  - Ashikaga T
AD  - Shiseido Co. Ltd, Quality Assessment Centre, Yokohama, Kanagawa, Japan. 
      takao.ashikaga@to.shiseido.co.jp
FAU - Sakaguchi, Hitoshi
AU  - Sakaguchi H
FAU - Sono, Sakiko
AU  - Sono S
FAU - Kosaka, Nanae
AU  - Kosaka N
FAU - Ishikawa, Makie
AU  - Ishikawa M
FAU - Nukada, Yuko
AU  - Nukada Y
FAU - Miyazawa, Masaaki
AU  - Miyazawa M
FAU - Ito, Yuichi
AU  - Ito Y
FAU - Nishiyama, Naohiro
AU  - Nishiyama N
FAU - Itagaki, Hiroshi
AU  - Itagaki H
LA  - eng
PT  - Journal Article
PL  - England
TA  - Altern Lab Anim
JT  - Alternatives to laboratory animals : ATLA
JID - 8110074
RN  - 0 (Antigens, CD)
RN  - 0 (Organic Chemicals)
SB  - IM
MH  - Animal Testing Alternatives/*methods/standards
MH  - Animals
MH  - Antigens, CD/drug effects/immunology
MH  - Cell Culture Techniques/methods
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Humans
MH  - Immunization
MH  - *Local Lymph Node Assay
MH  - Lymph Nodes/immunology
MH  - Organic Chemicals/pharmacology
MH  - Predictive Value of Tests
MH  - Skin/immunology
MH  - Skin Tests/*methods
EDAT- 2010/09/09 06:00
MHDA- 2010/11/17 06:00
CRDT- 2010/09/09 06:00
PHST- 2010/09/09 06:00 [entrez]
PHST- 2010/09/09 06:00 [pubmed]
PHST- 2010/11/17 06:00 [medline]
AID - 10.1177/026119291003800403 [doi]
PST - ppublish
SO  - Altern Lab Anim. 2010 Aug;38(4):275-84. doi: 10.1177/026119291003800403.