PMID- 20825428
OWN - NLM
STAT- MEDLINE
DCOM- 20111013
LR  - 20211020
IS  - 1365-2222 (Electronic)
IS  - 0954-7894 (Print)
IS  - 0954-7894 (Linking)
VI  - 41
IP  - 1
DP  - 2011 Jan
TI  - Dendritic cell and T cell responses in children with food allergy.
PG  - 61-71
LID - 10.1111/j.1365-2222.2010.03606.x [doi]
AB  - BACKGROUND: Food allergy (FA) and eosinophilic oesophagitis (EE) are increasingly 
      common clinical problems. Dendritic cells (DCs) are key regulators of the 
      sensitization and effector phases of allergic immune responses, but their role in 
      these diseases is largely unknown. OBJECTIVE: To evaluate for alterations in the 
      phenotype and function of DCs in children with IgE-mediated milk allergy or EE 
      compared with their non-affected siblings. METHODS: Plasmacytoid DCs (pDCs) and 
      myeloid DCs (mDCs) were prepared from peripheral blood of children with milk 
      allergy (FA), EE, and non-affected siblings (CON). Purified pDCs and mDCs were 
      cultured alone or with autologous CD4(+) lymphocytes. Cytokine levels in plasma, 
      or culture supernatants following stimulation, were measured using multiplex 
      array immunoassay. Cell-surface molecule expression was determined by flow 
      cytometry. RESULTS: DCs from FA subjects produced greater levels of 
      pro-inflammatory cytokines (IL-6, TNF-alpha), granulocyte macrophage-colony forming 
      factor, and mDC-derived IL-10 compared with controls following allergen exposure. 
      T(H) 2 but not T(H) 1 cytokines were spontaneously produced in DC-CD4(+) T cell 
      co-cultures from children with FA and were not significantly increased after 
      stimulation with milk extract, suggesting an ongoing activation in vivo. This 
      hypothesis was further supported by evidence for elevated IL-5 and IL-13 protein 
      in the plasma of children with both FA and EE. The only significant DC phenotypic 
      differences were: (1) reduced levels of CD80 in EE subjects and (2) FcvarepsilonRI 
      expression that correlated with serum IgE levels in both groups of subjects. 
      CONCLUSION: This study suggests that DCs from children with FA and EE produce 
      more pro-inflammatory cytokines, and that their CD4(+) T cells are spontaneously 
      activated to produce T(H) 2 cytokines in the presence of FcvarepsilonRI-bearing DCs.
CI  - (c) 2010 Blackwell Publishing Ltd.
FAU - Frischmeyer-Guerrerio, P A
AU  - Frischmeyer-Guerrerio PA
AD  - Department of Pediatrics, Division of Pediatric Allergy and Immunology, Johns 
      Hopkins Children's Center, Baltimore, MD, USA. pfrisch1@jhmi.edu
FAU - Guerrerio, A L
AU  - Guerrerio AL
FAU - Chichester, K L
AU  - Chichester KL
FAU - Bieneman, A P
AU  - Bieneman AP
FAU - Hamilton, R A
AU  - Hamilton RA
FAU - Wood, R A
AU  - Wood RA
FAU - Schroeder, J T
AU  - Schroeder JT
LA  - eng
GR  - R21 AI079853/AI/NIAID NIH HHS/United States
GR  - U19 AI070345/AI/NIAID NIH HHS/United States
GR  - T32AI007007/AI/NIAID NIH HHS/United States
GR  - T32 AI007007/AI/NIAID NIH HHS/United States
GR  - R21 AI079853-02/AI/NIAID NIH HHS/United States
GR  - U19AI070345-01/AI/NIAID NIH HHS/United States
GR  - R21 AI079853-01/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20100905
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
SB  - IM
MH  - Adolescent
MH  - Child
MH  - Child, Preschool
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Food Hypersensitivity/*immunology
MH  - Humans
MH  - Male
MH  - T-Lymphocytes/*immunology
MH  - Young Adult
PMC - PMC3006008
MID - NIHMS225275
COIS- The authors have no conflicts of interest to report.
EDAT- 2010/09/10 06:00
MHDA- 2011/10/14 06:00
PMCR- 2012/01/01
CRDT- 2010/09/10 06:00
PHST- 2010/09/10 06:00 [entrez]
PHST- 2010/09/10 06:00 [pubmed]
PHST- 2011/10/14 06:00 [medline]
PHST- 2012/01/01 00:00 [pmc-release]
AID - CEA3606 [pii]
AID - 10.1111/j.1365-2222.2010.03606.x [doi]
PST - ppublish
SO  - Clin Exp Allergy. 2011 Jan;41(1):61-71. doi: 10.1111/j.1365-2222.2010.03606.x. 
      Epub 2010 Sep 5.