PMID- 20826644
OWN - NLM
STAT- MEDLINE
DCOM- 20110210
LR  - 20211020
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Print)
IS  - 0095-1137 (Linking)
VI  - 48
IP  - 11
DP  - 2010 Nov
TI  - Real-time detection of influenza a, influenza B, and respiratory syncytial virus 
      a and B in respiratory specimens by use of nanoparticle probes.
PG  - 3997-4002
LID - 10.1128/JCM.01118-10 [doi]
AB  - Seasonal epidemics of influenza and respiratory syncytial virus are responsible 
      for significant morbidity and mortality worldwide. Infrequently, novel or 
      reemergent strains of influenza A virus have caused rapid, severe global 
      pandemics resulting in millions of fatalities. The ability to efficiently and 
      accurately detect and differentiate respiratory viruses is paramount for 
      effective treatment, infection control, and epidemiological surveillance. We 
      evaluated the ability of two FDA-cleared nucleic acid-based tests, the 
      semiautomated respiratory virus nucleic acid test (VRNAT) and the fully automated 
      respiratory virus nucleic acid test SP (RVNAT(SP)) (Nanosphere Inc., Northbrook, 
      IL) to detect influenza A virus, influenza B virus, and respiratory syncytial 
      virus A and B (RSV A/B) from clinical nasopharyngeal swab specimens. Detection of 
      viral RNA in both tests is based on nucleic acid amplification followed by 
      hybridization to capture probes immobilized on a glass slide. A novel technology 
      utilizing gold nanoparticle-conjugated probes is utilized to detect the presence 
      of captured target DNA. This microarray-based approach to detection has proven to 
      be more sensitive than the traditional culture/direct fluorescent-antibody assay 
      (DFA) method for detecting RSV and influenza viruses in clinical specimens, 
      including the novel 2009 H1N1 strain. Specifically, we report 98.0% sensitivity 
      and 96.5% specificity for the VRNAT compared to culture/DFA. Further, the VRNAT 
      detected virus in an additional 58% of specimens that were culture negative. 
      These data were confirmed using bidirectional sequencing. Evaluation of the fully 
      automated RVNAT(SP), which is built on the same detection technology as the VRNAT 
      but contains an updated processor enabling complete automation, revealed the two 
      tests to be functionally equivalent. Thus, the RVNAT(SP) is a fully automated 
      sample-to-result test capable of reliable detection of select respiratory viruses 
      directly from clinical specimens in 3.5 h.
FAU - Jannetto, Paul J
AU  - Jannetto PJ
AD  - Department of Pathology, Medical College of Wisconsin, Milwaukee, Wisconsin 
      53226, USA.
FAU - Buchan, Blake W
AU  - Buchan BW
FAU - Vaughan, Kimberly A
AU  - Vaughan KA
FAU - Ledford, Joellen S
AU  - Ledford JS
FAU - Anderson, Dennis K
AU  - Anderson DK
FAU - Henley, Donald C
AU  - Henley DC
FAU - Quigley, Neil B
AU  - Quigley NB
FAU - Ledeboer, Nathan A
AU  - Ledeboer NA
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100908
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Humans
MH  - Influenza A virus/*isolation & purification
MH  - Influenza B virus/*isolation & purification
MH  - Microarray Analysis/methods
MH  - *Nanoparticles
MH  - Nasopharynx/virology
MH  - *Oligonucleotide Probes
MH  - RNA, Viral/genetics/isolation & purification
MH  - Respiratory Syncytial Viruses/*isolation & purification
MH  - Respiratory Tract Infections/*virology
MH  - Sensitivity and Specificity
MH  - Virology/methods
MH  - Virus Diseases/*diagnosis/virology
PMC - PMC3020893
EDAT- 2010/09/10 06:00
MHDA- 2011/02/11 06:00
PMCR- 2011/05/01
CRDT- 2010/09/10 06:00
PHST- 2010/09/10 06:00 [entrez]
PHST- 2010/09/10 06:00 [pubmed]
PHST- 2011/02/11 06:00 [medline]
PHST- 2011/05/01 00:00 [pmc-release]
AID - JCM.01118-10 [pii]
AID - 1118-10 [pii]
AID - 10.1128/JCM.01118-10 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2010 Nov;48(11):3997-4002. doi: 10.1128/JCM.01118-10. Epub 2010 
      Sep 8.