PMID- 20826749
OWN - NLM
STAT- MEDLINE
DCOM- 20101019
LR  - 20100922
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 185
IP  - 7
DP  - 2010 Oct 1
TI  - The origin and maturity of dendritic cells determine the pattern of sphingosine 
      1-phosphate receptors expressed and required for efficient migration.
PG  - 4072-81
LID - 10.4049/jimmunol.1000568 [doi]
AB  - Dendritic cells (DCs) represent the most potent inducers of adaptive immune 
      responses. Depending on their activation phenotype, DCs drive naive T cells into 
      distinct differentiation pathways. To achieve this, DCs are present in virtually 
      all tissues where they sample Ag and migrate to the T cell areas of lymph nodes 
      (LNs) and spleen. Ample evidence exists demonstrating that sphingosine 
      1-phosphate (S1P) is an important modulator of these processes, exerting its 
      effects by binding to the S1P receptor S1P(1) and/or S1P(3). However, published 
      data are contradictory, in part. We show in this study that the expression 
      pattern, as well as the regulation of the S1P receptors, differs among in 
      vitro-generated DCs experiencing different kinds and duration of stimuli. 
      Moreover, the influence of S1P(1) and S1P(3) on the in vivo migration of maturing 
      DCs depends on the origin of these cells. Thus, in vitro-generated DCs require 
      S1P(1) and S1P(3) to accomplish this, whereas skin-derived DCs migrate unhindered 
      in the absence of S1P(3) but not when S1P(1) signaling is blocked. Migration of 
      lamina propria DCs to the mesenteric LNs depends on S1P(1) and S1P(3). In 
      contrast, relocation of maturing spleen-resident DCs to the T cell zone is 
      independent of S1P(1) and S1P(3). However, intrasplenic positioning of immature 
      DCs to the bridging channels depends on S1P(1) activity, with no noticeable 
      contribution of S1P(3). These observations reveal a tissue-dependent contribution 
      of S1P(3) to DC migration and suggest a fundamental role for S1P(1) for maturing 
      DCs migrating from periphery to draining LNs.
FAU - Rathinasamy, Anchana
AU  - Rathinasamy A
AD  - Institute of Immunology, Hannover Medical School, Hannover, Germany.
FAU - Czeloth, Niklas
AU  - Czeloth N
FAU - Pabst, Oliver
AU  - Pabst O
FAU - Forster, Reinhold
AU  - Forster R
FAU - Bernhardt, Gunter
AU  - Bernhardt G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100908
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Receptors, Lysosphingolipid)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/immunology
MH  - Cell Separation
MH  - Chemotaxis, Leukocyte/*immunology
MH  - Dendritic Cells/cytology/*immunology/metabolism
MH  - Flow Cytometry
MH  - Gene Expression
MH  - Immunohistochemistry
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mucous Membrane/cytology/immunology
MH  - Receptors, Lysosphingolipid/*immunology/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Skin/cytology/immunology
EDAT- 2010/09/10 06:00
MHDA- 2010/10/20 06:00
CRDT- 2010/09/10 06:00
PHST- 2010/09/10 06:00 [entrez]
PHST- 2010/09/10 06:00 [pubmed]
PHST- 2010/10/20 06:00 [medline]
AID - jimmunol.1000568 [pii]
AID - 10.4049/jimmunol.1000568 [doi]
PST - ppublish
SO  - J Immunol. 2010 Oct 1;185(7):4072-81. doi: 10.4049/jimmunol.1000568. Epub 2010 
      Sep 8.