PMID- 20828517
OWN - NLM
STAT- MEDLINE
DCOM- 20110201
LR  - 20130410
IS  - 1916-0216 (Electronic)
IS  - 1916-0208 (Linking)
VI  - 39
IP  - 5
DP  - 2010 Oct
TI  - Immunohistochemical localization of glutathione s-transferase isoenzymes (gsta, 
      Gstp, Gstm4, And Gstt1) and tumour marker p53 in matched tissue from normal 
      larynx and laryngeal carcinoma: correlations with prognostic factors.
PG  - 542-50
AB  - OBJECTIVE: The immunohistochemical staining characteristics of glutathione 
      S-transferase (GST) alpha (GSTA), pi (GSTP), mu (GSTM4), and theta (GSTT1) and 
      P53 were investigated in laryngeal squamous cell carcinoma (LSCC) cases and 
      normal laryngeal tissue from 46 patients. The relationships between expression of 
      the GST isoenzymes and some clinicopathologic features were also examined. 
      PATIENTS AND METHODS: For immunohistochemical studies, tissues from 46 patients 
      with LSCC at the Dr. Lutfi Kirdar Kartal Education and Research Hospital were 
      used. The relationship between expression of the GST isoenzymes and P53 in normal 
      and tumour tissue was analyzed using the Wilcoxon signed rank test. The 
      correlation between GST isoenzymes and P53 and clinicopathologic data was also 
      examined using the Spearman rank test. RESULTS: When the normal and tumour 
      tissues of these cases were compared according to their staining intensity and 
      percentage of positive staining, GSTA expression in normal cells was 
      significantly higher than in tumour cells, and GSTP and P53 expression was higher 
      in tumour cells (p < .05). GSTM and GSTT1 expression was higher in normal cells; 
      however, the statistical significance was low (p > .05). There was no correlation 
      between P53 and GST expression in patients with LSCC. When the 
      immunohistochemical results of GST isoenzymes and P53 were correlated with the 
      clinical parameters, GSTA expression was increased in poorly differentiated 
      laryngeal tumour, but GSTM4 and GSTT1 expression was decreased (p < .05). 
      CONCLUSION: According to these results, GST-A, -P, and T1 and P53 were important 
      in the diagnosis of LSCC.
FAU - Sedat, Aydin
AU  - Sedat A
AD  - Department ofOtolaryngology, Head and Neck Surgery, Gu lhane Military 
      MedicalAcademy, Ankara, Turkey. sedataydin63@yahoo.com
FAU - Serpil, Oguztuzun
AU  - Serpil O
FAU - Nurdan, Gurbuz
AU  - Nurdan G
FAU - Aylin, Gul
AU  - Aylin G
FAU - Arif, Sanli
AU  - Arif S
FAU - Muzeyyen, Ozhavzali
AU  - Muzeyyen O
FAU - Bulent, Satar
AU  - Bulent S
FAU - Sezen, Ozan
AU  - Sezen O
FAU - Nimet, Karadayi
AU  - Nimet K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Otolaryngol Head Neck Surg
JT  - Journal of otolaryngology - head & neck surgery = Le Journal 
      d'oto-rhino-laryngologie et de chirurgie cervico-faciale
JID - 101479544
RN  - 0 (Tumor Suppressor Protein p53)
RN  - EC 2.5.1.- (glutathione S-transferase T1)
RN  - EC 2.5.1.18 (GSTA1 protein, human)
RN  - EC 2.5.1.18 (GSTM4 protein, human)
RN  - EC 2.5.1.18 (Glutathione S-Transferase pi)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Glutathione S-Transferase pi/biosynthesis
MH  - Glutathione Transferase/*biosynthesis
MH  - Humans
MH  - Immunohistochemistry/*methods
MH  - Laryngeal Neoplasms/*metabolism/pathology
MH  - Larynx/cytology/*metabolism
MH  - Prognosis
MH  - Tumor Suppressor Protein p53/*biosynthesis
EDAT- 2010/09/11 06:00
MHDA- 2011/02/02 06:00
CRDT- 2010/09/11 06:00
PHST- 2010/09/11 06:00 [entrez]
PHST- 2010/09/11 06:00 [pubmed]
PHST- 2011/02/02 06:00 [medline]
PST - ppublish
SO  - J Otolaryngol Head Neck Surg. 2010 Oct;39(5):542-50.