PMID- 20828738
OWN - NLM
STAT- MEDLINE
DCOM- 20120106
LR  - 20171116
IS  - 1095-8673 (Electronic)
IS  - 0022-4804 (Linking)
VI  - 171
IP  - 2
DP  - 2011 Dec
TI  - Exosomes derived from immature bone marrow dendritic cells induce tolerogenicity 
      of intestinal transplantation in rats.
PG  - 826-32
LID - 10.1016/j.jss.2010.05.021 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) secrete exosomes bearing major 
      histocompatibility complex I and II (MHC I /II) and co-stimulatory molecules, and 
      play a critical role in immune regulation. Because immature DCs can induce T-cell 
      tolerance in vitro and in vivo, we explored the possibility of using exosomes 
      derived from immature DCs (imDex) for the induction of intestinal transplant 
      tolerance in rats. MATERIALS AND METHODS: ImDex were purified from F344 rat bone 
      marrow immature DCs. The tolerizing capacities of imDex were analyzed in vitro 
      and in vivo using a F344-to-Wistar intestinal transplantation model. RESULTS: In 
      the context of a moderate level of MHC class II and a low co-stimulatory level 
      expression, imDex significantly suppressed the alloreactive T-cell response with 
      an increase in IL-10 in vitro. In vivo injection of the lower dose (20 mug) of 
      donor (but not recipient) imDex can significantly prolong the survival of 
      intestinal allografts. This effect was accompanied by a decrease in the 
      anti-donor cellular response, with a significant increase in IL-10. The 
      CD4+CD25+T cells percentage and FOXP3mRNA expression in splenic T-cells were also 
      significantly increased in imDex treatment recipients at five days after 
      transplantation. CONCLUSIONS: The results suggest that imDex can prolong the 
      intestinal allograft survival and may be a potential strategy to facilitate 
      induction of transplant tolerance.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Yang, Xiaojun
AU  - Yang X
AD  - Department of Gastrointestinal Surgery, First Affiliated Hospital of Nanjing 
      Medical University, Nanjing, Jiangsu Province, China.
FAU - Meng, Song
AU  - Meng S
FAU - Jiang, Hong
AU  - Jiang H
FAU - Zhu, Chunfu
AU  - Zhu C
FAU - Wu, Wenxi
AU  - Wu W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100608
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (CD4 Antigens)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Foxp3 protein, rat)
RN  - 0 (Interleukin-2 Receptor alpha Subunit)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Bone Marrow Cells/*immunology
MH  - CD4 Antigens/metabolism
MH  - Dendritic Cells/*immunology
MH  - Exosomes/*immunology
MH  - Forkhead Transcription Factors/metabolism
MH  - Graft Rejection/immunology/prevention & control
MH  - Graft Survival/*immunology
MH  - Immune Tolerance/*immunology
MH  - Interleukin-2 Receptor alpha Subunit/metabolism
MH  - Intestines/*transplantation
MH  - Male
MH  - Rats
MH  - Rats, Inbred F344
MH  - Spleen/cytology/immunology
MH  - T-Lymphocytes/immunology/metabolism
MH  - Transplantation, Homologous
EDAT- 2010/09/11 06:00
MHDA- 2012/01/10 06:00
CRDT- 2010/09/11 06:00
PHST- 2010/01/13 00:00 [received]
PHST- 2010/04/21 00:00 [revised]
PHST- 2010/05/11 00:00 [accepted]
PHST- 2010/09/11 06:00 [entrez]
PHST- 2010/09/11 06:00 [pubmed]
PHST- 2012/01/10 06:00 [medline]
AID - S0022-4804(10)00472-5 [pii]
AID - 10.1016/j.jss.2010.05.021 [doi]
PST - ppublish
SO  - J Surg Res. 2011 Dec;171(2):826-32. doi: 10.1016/j.jss.2010.05.021. Epub 2010 Jun 
      8.