PMID- 20830676
OWN - NLM
STAT- MEDLINE
DCOM- 20110328
LR  - 20131121
IS  - 1097-0290 (Electronic)
IS  - 0006-3592 (Linking)
VI  - 108
IP  - 2
DP  - 2011 Feb
TI  - Effects of the antimicrobial tylosin on the microbial community structure of an 
      anaerobic sequencing batch reactor.
PG  - 296-305
LID - 10.1002/bit.22934 [doi]
AB  - The effects of the antimicrobial tylosin on a methanogenic microbial community 
      were studied in a glucose-fed laboratory-scale anaerobic sequencing batch reactor 
      (ASBR) exposed to stepwise increases of tylosin (0, 1.67, and 167 mg/L). The 
      microbial community structure was determined using quantitative fluorescence in 
      situ hybridization (FISH) and phylogenetic analyses of bacterial 16S ribosomal 
      RNA (rRNA) gene clone libraries of biomass samples. During the periods without 
      tylosin addition and with an influent tylosin concentration of 1.67 mg/L, 16S 
      rRNA gene sequences related to Syntrophobacter were detected and the relative 
      abundance of Methanosaeta species was high. During the highest tylosin dose of 
      167 mg/L, 16S rRNA gene sequences related to Syntrophobacter species were not 
      detected and the relative abundance of Methanosaeta decreased considerably. 
      Throughout the experimental period, Propionibacteriaceae and high GC 
      Gram-positive bacteria were present, based on 16S rRNA gene sequences and FISH 
      analyses, respectively. The accumulation of propionate and subsequent reactor 
      failure after long-term exposure to tylosin are attributed to the direct 
      inhibition of propionate-oxidizing syntrophic bacteria closely related to 
      Syntrophobacter and the indirect inhibition of Methanosaeta by high propionate 
      concentrations and low pH.
CI  - (c) 2010 Wiley Periodicals, Inc.
FAU - Shimada, Toshio
AU  - Shimada T
AD  - Department of Civil and Environmental Engineering, University of Illinois at 
      Urbana-Champaign, USA.
FAU - Li, Xu
AU  - Li X
FAU - Zilles, Julie L
AU  - Zilles JL
FAU - Morgenroth, Eberhard
AU  - Morgenroth E
FAU - Raskin, Lutgarde
AU  - Raskin L
LA  - eng
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PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - OP0UW79H66 (Methane)
RN  - YEF4JXN031 (Tylosin)
SB  - IM
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Archaea/*drug effects/isolation & purification/metabolism
MH  - Bacteria, Anaerobic/*drug effects/isolation & purification/metabolism
MH  - *Biodiversity
MH  - Bioreactors/*microbiology
MH  - Cluster Analysis
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Methane/*metabolism
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Tylosin/*pharmacology
EDAT- 2010/09/11 06:00
MHDA- 2011/03/29 06:00
CRDT- 2010/09/11 06:00
PHST- 2010/09/11 06:00 [entrez]
PHST- 2010/09/11 06:00 [pubmed]
PHST- 2011/03/29 06:00 [medline]
AID - 10.1002/bit.22934 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2011 Feb;108(2):296-305. doi: 10.1002/bit.22934.