PMID- 20831580
OWN - NLM
STAT- MEDLINE
DCOM- 20101230
LR  - 20100913
IS  - 1600-0722 (Electronic)
IS  - 0909-8836 (Linking)
VI  - 118
IP  - 5
DP  - 2010 Oct
TI  - Molecular epidemiology and spatial distribution of Selenomonas spp. in 
      subgingival biofilms.
PG  - 466-74
LID - 10.1111/j.1600-0722.2010.00765.x [doi]
AB  - The aetiology of periodontal disease has been a field of intensive research in 
      the past decades. Along with a variety of other putative pathogens, different 
      members of the genus Selenomonas have repeatedly been associated with both 
      generalized aggressive periodontitis and chronic periodontitis. For the present 
      study, a specific oligonucleotide probe targeting the majority of all oral 
      Selenomonas spp. was designed. Their prevalence was determined, using dot-blot 
      hybridization, in a total of 742 subgingival samples collected from patients with 
      generalized aggressive (n=62) and chronic periodontitis (n=82), and from 
      periodontitis-resistant subjects (n=19). In addition, fluorescence in situ 
      hybridization (FISH) and electron microscopy were performed to analyze the 
      spatial arrangement of Selenomonas in subgingival biofilms collected from 
      patients with generalized aggressive periodontitis. In the samples from patients, 
      Selenomonas spp. showed a lower prevalence in both diseased groups compared with 
      other putative pathogens, and a relatively high prevalence in the 
      periodontitis-resistant group. Consequently, Selenomonas spp. do not seem to be 
      suitable diagnostic marker organisms for periodontal disease. By contrast, FISH 
      and electron microscopic analysis of periodontal carriers revealed that 
      Selenomonas spp. appeared in large numbers in all parts of the collected biofilms 
      and seemed, if present in a site from patients, to make a relevant contribution 
      to their structural organization.
CI  - (c) 2010 Eur J Oral Sci.
FAU - Drescher, Julia
AU  - Drescher J
AD  - Institut fur Mikrobiologie und Hygiene, Charite- Universitatsmedizin Berlin, 
      Berlin, Germany.
FAU - Schlafer, Sebastian
AU  - Schlafer S
FAU - Schaudinn, Christoph
AU  - Schaudinn C
FAU - Riep, Birgit
AU  - Riep B
FAU - Neumann, Konrad
AU  - Neumann K
FAU - Friedmann, Anton
AU  - Friedmann A
FAU - Petrich, Annett
AU  - Petrich A
FAU - Gobel, Ulf B
AU  - Gobel UB
FAU - Moter, Annette
AU  - Moter A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Oral Sci
JT  - European journal of oral sciences
JID - 9504563
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aggressive Periodontitis/epidemiology/*microbiology
MH  - Biofilms
MH  - Carrier State/microbiology
MH  - Case-Control Studies
MH  - Chronic Periodontitis/epidemiology/*microbiology
MH  - DNA, Bacterial/genetics
MH  - Dental Plaque/*microbiology
MH  - Female
MH  - Host Specificity
MH  - Humans
MH  - Immunoblotting
MH  - In Situ Hybridization, Fluorescence
MH  - Logistic Models
MH  - Male
MH  - Middle Aged
MH  - Molecular Epidemiology
MH  - RNA, Ribosomal, 16S/genetics
MH  - Selenomonas/classification/*genetics
EDAT- 2010/09/14 06:00
MHDA- 2010/12/31 06:00
CRDT- 2010/09/14 06:00
PHST- 2010/09/14 06:00 [entrez]
PHST- 2010/09/14 06:00 [pubmed]
PHST- 2010/12/31 06:00 [medline]
AID - 10.1111/j.1600-0722.2010.00765.x [doi]
PST - ppublish
SO  - Eur J Oral Sci. 2010 Oct;118(5):466-74. doi: 10.1111/j.1600-0722.2010.00765.x.