PMID- 20832111
OWN - NLM
STAT- MEDLINE
DCOM- 20110118
LR  - 20181201
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 31
IP  - 33
DP  - 2010 Nov
TI  - Phenotype and functional analysis of human monocytes-derived dendritic cells 
      loaded with a carbosilane dendrimer.
PG  - 8749-58
LID - 10.1016/j.biomaterials.2010.07.093 [doi]
AB  - Dendritic cells (DCs) play a major role in development of cell-mediated 
      immunotherapy due to their unique role in linking innate and adaptive immunities. 
      In spite of improvement in this area, strategies employing ex vivo generated DCs 
      have shown limited efficacy in clinical trials. Dendrimers have been proposed as 
      new carriers for drug delivery in aim to ameliorate DCs antigen loading that is a 
      pivotal point in DCs approaches. In this study, we have investigated the 
      phenotypic and functional characteristics of human monocytes-derived dendritic 
      cells after HIV-derived peptides uptake in vitro. We have found that iDCs and 
      mDCs were able to capture efficiently water soluble carbosilane (CBS) dendrimer 2 
      G-NN16 and did not induce changes in maturation markers levels at the DCs 
      surface. Therefore, CBS 2 G-NN16-loaded mDCs migrated as efficiently as unloaded 
      DCs towards CCL19 or CCL21. Furthermore, DCs viability, activation of allogenic 
      naive CD4 + T cells by mDCs and secretion of cytokines were not significantly 
      changed by 2 G-NN16 loading. Summing up, our data indicate that CBS 2 G-NN16 has 
      no negative effects on the pivotal properties of DCs in vitro. It should 
      therefore be feasible to further develop this antigen loading strategy for 
      clinical use in immunotherapy against viral infections.
CI  - Copyright (c) 2010 Elsevier Ltd. All rights reserved.
FAU - Pion, Marjorie
AU  - Pion M
AD  - Laboratorio Inmuno-Biologia Molecular, Hospital General Universitario Gregorio 
      Maranon, Madrid 28007, Spain.
FAU - Serramia, Maria J
AU  - Serramia MJ
FAU - Diaz, Laura
AU  - Diaz L
FAU - Bryszewska, Maria
AU  - Bryszewska M
FAU - Gallart, Teresa
AU  - Gallart T
FAU - Garcia, Felipe
AU  - Garcia F
FAU - Gomez, Rafael
AU  - Gomez R
FAU - de la Mata, Francisco J
AU  - de la Mata FJ
FAU - Munoz-Fernandez, Maria A
AU  - Munoz-Fernandez MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100915
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
RN  - 0 (Dendrimers)
RN  - 0 (Peptides)
RN  - 0 (Silanes)
RN  - 0 (carbosilane)
SB  - IM
MH  - Biomarkers/metabolism
MH  - Cell Death/drug effects
MH  - Cell Differentiation/drug effects
MH  - Cell Membrane/drug effects/metabolism
MH  - Cell Movement/drug effects
MH  - Cytokines/metabolism
MH  - Dendrimers/*pharmacology/toxicity
MH  - Dendritic Cells/*cytology/*drug effects
MH  - Humans
MH  - Lymphocyte Activation/drug effects
MH  - Monocytes/*cytology
MH  - Peptides/metabolism
MH  - Phenotype
MH  - Silanes/*pharmacology/toxicity
EDAT- 2010/09/14 06:00
MHDA- 2011/01/19 06:00
CRDT- 2010/09/14 06:00
PHST- 2010/03/26 00:00 [received]
PHST- 2010/07/23 00:00 [accepted]
PHST- 2010/09/14 06:00 [entrez]
PHST- 2010/09/14 06:00 [pubmed]
PHST- 2011/01/19 06:00 [medline]
AID - S0142-9612(10)00965-8 [pii]
AID - 10.1016/j.biomaterials.2010.07.093 [doi]
PST - ppublish
SO  - Biomaterials. 2010 Nov;31(33):8749-58. doi: 10.1016/j.biomaterials.2010.07.093. 
      Epub 2010 Sep 15.