PMID- 20833144
OWN - NLM
STAT- MEDLINE
DCOM- 20101130
LR  - 20211020
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 401
IP  - 1
DP  - 2010 Oct 8
TI  - Cytochrome c oxidase loses catalytic activity and structural integrity during the 
      aging process in Drosophila melanogaster.
PG  - 64-8
LID - 10.1016/j.bbrc.2010.09.009 [doi]
AB  - The hypothesis, that structural deterioration of cytochrome c oxidase (CcO) is a 
      causal factor in the age-related decline in mitochondrial respiratory activity 
      and an increase in H(2)O(2) generation, was tested in Drosophila melanogaster. CcO 
      activity and the levels of seven different nuclear DNA-encoded CcO subunits were 
      determined at three different stages of adult life, namely, young-, middle-, and 
      old-age. CcO activity declined progressively with age by 33%. Western blot 
      analysis, using antibodies specific to Drosophila CcO subunits IV, Va, Vb, VIb, 
      VIc, VIIc, and VIII, indicated that the abundance these polypeptides decreased, 
      ranging from 11% to 40%, during aging. These and previous results suggest that 
      CcO is a specific intra-mitochondrial site of age-related deterioration, which 
      may have a broad impact on mitochondrial physiology.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Ren, Jian-Ching
AU  - Ren JC
AD  - Department of Pharmacology and Pharmaceutical Sciences, University of Southern 
      California, Los Angeles, CA 90033, USA.
FAU - Rebrin, Igor
AU  - Rebrin I
FAU - Klichko, Vladimir
AU  - Klichko V
FAU - Orr, William C
AU  - Orr WC
FAU - Sohal, Rajindar S
AU  - Sohal RS
LA  - eng
GR  - R01 AG007657/AG/NIA NIH HHS/United States
GR  - R01 AG7657/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20100915
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Protein Subunits)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Aging/*metabolism
MH  - Animals
MH  - Catalysis
MH  - Drosophila melanogaster/enzymology/*physiology
MH  - Electron Transport Complex IV/chemistry/*metabolism
MH  - Protein Subunits/chemistry/metabolism
PMC - PMC2964050
MID - NIHMS241934
EDAT- 2010/09/14 06:00
MHDA- 2010/12/14 06:00
PMCR- 2011/10/08
CRDT- 2010/09/14 06:00
PHST- 2010/08/19 00:00 [received]
PHST- 2010/09/06 00:00 [accepted]
PHST- 2010/09/14 06:00 [entrez]
PHST- 2010/09/14 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
PHST- 2011/10/08 00:00 [pmc-release]
AID - S0006-291X(10)01684-0 [pii]
AID - 10.1016/j.bbrc.2010.09.009 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2010 Oct 8;401(1):64-8. doi: 
      10.1016/j.bbrc.2010.09.009. Epub 2010 Sep 15.