PMID- 20833212
OWN - NLM
STAT- MEDLINE
DCOM- 20110225
LR  - 20211020
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 154
IP  - 1-2
DP  - 2010 Dec
TI  - Identification of dispensable nucleotide sequence in 3' untranslated region of 
      porcine reproductive and respiratory syndrome virus.
PG  - 38-47
LID - 10.1016/j.virusres.2010.08.027 [doi]
AB  - The 3' untranslated region (UTR) of porcine arterivirus genome plays a pivotal 
      role for virus replication, yet the properties of 3' UTR remain largely 
      undefined. We conducted site-directed mutagenesis to the 3' UTR of the type II 
      porcine reproductive and respiratory syndrome virus (PRRSV). Serial deletions of 
      the 3' UTR showed that at least 40 nucleotides immediately following the ORF7 
      stop codon were dispensable for the viability of PRRSV in cultured cells. We then 
      constructed a chimeric PRRSV cDNA clone using type II PRRSV as the backbone 
      containing the 3' UTR from the type I PRRSV. The chimeric virus was viable and 
      shared similar properties with the parental virus. Our results provided the first 
      description of the 40nt dispensable region in type I PRRSV 3' UTR, and further 
      predicted structure demonstrated that the high-order structure of 3' UTR might 
      play significant roles in its function.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Sun, Zhi
AU  - Sun Z
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
FAU - Liu, Changlong
AU  - Liu C
FAU - Tan, Feifei
AU  - Tan F
FAU - Gao, Fei
AU  - Gao F
FAU - Liu, Ping
AU  - Liu P
FAU - Qin, Aijian
AU  - Qin A
FAU - Yuan, Shishan
AU  - Yuan S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20100915
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (3' Untranslated Regions)
SB  - IM
MH  - *3' Untranslated Regions
MH  - Animals
MH  - Cell Line
MH  - Microbial Viability
MH  - Mutagenesis, Site-Directed
MH  - Nucleic Acid Conformation
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/physiology
MH  - Recombination, Genetic
MH  - Sequence Deletion
MH  - Swine
MH  - *Virus Replication
PMC - PMC7114379
EDAT- 2010/09/14 06:00
MHDA- 2011/02/26 06:00
PMCR- 2010/09/15
CRDT- 2010/09/14 06:00
PHST- 2010/03/29 00:00 [received]
PHST- 2010/08/18 00:00 [revised]
PHST- 2010/08/27 00:00 [accepted]
PHST- 2010/09/14 06:00 [entrez]
PHST- 2010/09/14 06:00 [pubmed]
PHST- 2011/02/26 06:00 [medline]
PHST- 2010/09/15 00:00 [pmc-release]
AID - S0168-1702(10)00324-2 [pii]
AID - 10.1016/j.virusres.2010.08.027 [doi]
PST - ppublish
SO  - Virus Res. 2010 Dec;154(1-2):38-47. doi: 10.1016/j.virusres.2010.08.027. Epub 
      2010 Sep 15.