PMID- 20835848
OWN - NLM
STAT- MEDLINE
DCOM- 20110519
LR  - 20211020
IS  - 1573-0832 (Electronic)
IS  - 0301-486X (Linking)
VI  - 171
IP  - 3
DP  - 2011 Mar
TI  - Cyclosporin A treatment and decreased fungal load/antigenemia in experimental 
      murine paracoccidioidomycosis.
PG  - 161-9
LID - 10.1007/s11046-010-9359-5 [doi]
AB  - Paracoccidioidomycosis (PCM) is a systemic mycosis caused by the fungus 
      Paracoccidioides brasiliensis (Pb). The cyclosporin A (CsA) is an 
      immunosuppressant drug that inhibits calcineurin and has been described as a 
      potential antifungal drug. The present study investigated the effect of CsA on 
      the immune response, fungal load/antigenemia in experimental murine PCM. It was 
      used four groups of BALB/c mice: (a) infected with 1 x 10(5) Pb18 yeast cells (Pb), 
      (b) infected and treated with CsA every other day 10 mg/kg of CsA (s.c.) during 
      30 days (Pb/CsA), (c) treated with CsA (CsA) and (d) no infected/treated (PBS). 
      The immune response was evaluated by lymphocyte proliferation, DTH assays to 
      exoAgs, ELISA for IgG anti-gp43 (specific immune responses) and cytokine serum 
      levels (IFN-gamma, TNF-alpha, IL-4 and IL-10). Fungal load was determined by lung 
      colony-forming units (CFU) counts, lung and liver histopathology analysis and 
      antigenemia determined by inhibition-ELISA. As expected, CsA was able to inhibit 
      the specific cellular and humoral immune response (P < 0.05), with decrease in 
      serum IFN-gamma, TNF-alpha and IL-4 levels (P < 0.05). Cyclosporin A treatment also 
      resulted in significantly decreased lung Pb CFU (P < 0.05) as well as a lower 
      number of yeasts in the lung and liver (P < 0.05) by histopathology. In 
      concordance, the decreased antigenemia was observed in Pb/CsA group (P < 0.05). 
      In conclusion, even with immunosuppressive action, treatment with CsA results in 
      decreased lung fungal load/antigenemia in experimental PCM in BALB/c mice. 
      Further study is required to determine whether this represents less severe 
      disease or protection by CsA.
FAU - Massuda, T Y C
AU  - Massuda TY
AD  - Department of Pathology Sciences, State University of Londrina, CCB, Universidade 
      Estadual de Londrina, Campus Universitario, Londrina, PR 86051-970, Brazil.
FAU - Nagashima, L A
AU  - Nagashima LA
FAU - Leonello, P C
AU  - Leonello PC
FAU - Kaminami, M S
AU  - Kaminami MS
FAU - Mantovani, M S
AU  - Mantovani MS
FAU - Sano, A
AU  - Sano A
FAU - Uno, J
AU  - Uno J
FAU - Venancio, E J
AU  - Venancio EJ
FAU - Camargo, Z P
AU  - Camargo ZP
FAU - Itano, E N
AU  - Itano EN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100911
PL  - Netherlands
TA  - Mycopathologia
JT  - Mycopathologia
JID - 7505689
RN  - 0 (43 kDa protein, Paracoccidioides)
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Fungal Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 83HN0GTJ6D (Cyclosporine)
SB  - IM
MH  - Animals
MH  - Antibodies, Fungal/blood
MH  - Antigens, Fungal/blood/immunology
MH  - Colony Count, Microbial
MH  - Cyclosporine/immunology/*therapeutic use
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fungal Proteins/blood/immunology
MH  - Glycoproteins/blood/immunology
MH  - Hypersensitivity, Delayed/immunology
MH  - Immunoglobulin G/blood
MH  - Interferon-gamma/blood
MH  - Interleukin-10/blood
MH  - Interleukin-4/blood
MH  - Liver/microbiology/pathology
MH  - Lung/*microbiology/pathology
MH  - Lymphocyte Activation
MH  - Lymphocytes/immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - *Paracoccidioides/drug effects/growth & development/immunology
MH  - Paracoccidioidomycosis/*drug therapy/immunology/microbiology/pathology
MH  - Tumor Necrosis Factor-alpha/blood
EDAT- 2010/09/14 06:00
MHDA- 2011/05/20 06:00
CRDT- 2010/09/14 06:00
PHST- 2010/06/01 00:00 [received]
PHST- 2010/08/25 00:00 [accepted]
PHST- 2010/09/14 06:00 [entrez]
PHST- 2010/09/14 06:00 [pubmed]
PHST- 2011/05/20 06:00 [medline]
AID - 10.1007/s11046-010-9359-5 [doi]
PST - ppublish
SO  - Mycopathologia. 2011 Mar;171(3):161-9. doi: 10.1007/s11046-010-9359-5. Epub 2010 
      Sep 11.