PMID- 20837561
OWN - NLM
STAT- MEDLINE
DCOM- 20110609
LR  - 20220311
IS  - 1477-0393 (Electronic)
IS  - 0748-2337 (Linking)
VI  - 27
IP  - 2
DP  - 2011 Mar
TI  - Thymoquinone reestablishes spermatogenesis after testicular injury caused by 
      chronic toluene exposure in rats.
PG  - 155-66
LID - 10.1177/0748233710382541 [doi]
AB  - The aim of this study was designed to evaluate the possible protective effects of 
      thymoquinone (TQ) on the spermatogenesis after testicular injury caused by 
      chronic toluene exposure in rats. The rats were randomly allotted into one of 
      three experimental groups: control, toluene-treated and toluene treated with TQ; 
      each group contained 10 animals. Control group received 1 mL serum physiologic 
      and toluene treatment was performed by inhalation of 3000 ppm toluene, in an 
      8-hour/day and 6-day/week order for 12 weeks. The rats in TQ-treated group was 
      given TQ (50 mg/kg body weight) once a day orally for 12 weeks starting just 
      after toluene exposure. Tissue samples were obtained for histopathological 
      investigation. To date, no histopathological changes of testis in rats after 
      chronic toluene exposure by TQ treatment have been reported. Spermatogenesis and 
      mean seminiferous tubule diameter (MSTD) were significantly decreased in toluene 
      treated groups when compared to the control group. Furthermore, the TQ-treated 
      animals showed an improved histological appearance in toluene-treated group. Our 
      data indicate a significant reduction in the activity of in situ identification 
      of apoptosis using terminal dUTP nick end-labeling (TUNEL), endothelial nitric 
      oxide synthase (eNOS), inducible nitric oxide synthase (iNOS) and there was a 
      rise in the expression of proliferating cell nuclear antigen (PCNA) in testis 
      tissues of the toluene-treated group with TQ therapy. Electron microscopy of the 
      testes of the rats demonstrated that pretreatment with TQ was particularly 
      effective in preventing the mitochondrial degeneration, dilatation of smooth 
      endoplasmic reticulum (SER) and enlarged intercellular spaces in both Sertoli and 
      spermatid cells in the toluene-treated animals. We believe that further 
      preclinical research into the utility of TQ may indicate its usefulness as a 
      potential treatment on the spermatogenesis after testicular injury caused by 
      chronic toluene exposure in rats.
FAU - Kanter, Mehmet
AU  - Kanter M
AD  - Department of Histology and Embryology, Faculty of Medicine, Trakya University, 
      Edirne, Turkey. mkanter65@yahoo.com
LA  - eng
PT  - Journal Article
DEP - 20100913
PL  - England
TA  - Toxicol Ind Health
JT  - Toxicology and industrial health
JID - 8602702
RN  - 0 (Benzoquinones)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 3FPU23BG52 (Toluene)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
RN  - EC 1.14.13.39 (Nos2 protein, rat)
RN  - EC 1.14.13.39 (Nos3 protein, rat)
RN  - O60IE26NUF (thymoquinone)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Benzoquinones/*pharmacology
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Microscopy, Electron
MH  - Nitric Oxide Synthase Type II/metabolism
MH  - Nitric Oxide Synthase Type III/metabolism
MH  - Proliferating Cell Nuclear Antigen/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Seminiferous Tubules/drug effects/pathology
MH  - Spermatogenesis/*drug effects
MH  - Testicular Diseases/chemically induced/*drug therapy
MH  - Testis/metabolism/physiopathology
MH  - Toluene/*toxicity
EDAT- 2010/09/15 06:00
MHDA- 2011/06/10 06:00
CRDT- 2010/09/15 06:00
PHST- 2010/09/15 06:00 [entrez]
PHST- 2010/09/15 06:00 [pubmed]
PHST- 2011/06/10 06:00 [medline]
AID - 0748233710382541 [pii]
AID - 10.1177/0748233710382541 [doi]
PST - ppublish
SO  - Toxicol Ind Health. 2011 Mar;27(2):155-66. doi: 10.1177/0748233710382541. Epub 
      2010 Sep 13.