PMID- 20839998
OWN - NLM
STAT- MEDLINE
DCOM- 20110726
LR  - 20180629
IS  - 1477-2566 (Electronic)
IS  - 1465-3249 (Linking)
VI  - 13
IP  - 2
DP  - 2011 Feb
TI  - Human marrow-isolated adult multilineage-inducible (MIAMI) cells protect against 
      peripheral vascular ischemia in a mouse model.
PG  - 179-92
LID - 10.3109/14653249.2010.515579 [doi]
AB  - BACKGROUND AIMS: The treatment of peripheral vascular disease (PVD) with stem 
      cells potentially offers a promising strategy. We tested marrow-isolated adult 
      multilineage-inducible (MIAMI) cells to induce neovascularization in a mouse 
      model of critical hindlimb ischemia (CLI). METHODS: CLI was induced in the right 
      hindlimb of Balb/C mice. One million MIAMI cells, normally grown at 3% O(2), were 
      injected in the adductor muscle along the ischemic region. All animals (n = 11 
      per group) were immunosuppressed with cyclosporine daily for the entire period. 
      Human foreskin fibroblast (HFF) cells and phosphate-buffered saline (PBS) were 
      used as controls. Blood perfusion in the ischemic right and non-ischemic left 
      hindlimbs was measured. RESULTS: Compared with animals receiving HFF cells or 
      PBS, MIAMI cells significantly improved blood perfusion, necrosis and 
      inflammation in the ischemic limb. A fraction of injected MIAMI cells expressed 
      CD31 and von Willebrand factor (vWF). MIAMI cells in vitro, under pro-angiogenic 
      growth conditions, differentiated into endothelial-like cells and expressed 
      endothelial markers such as CD31 and vWF, determined by quantitative reverse 
      transcriptase-polymerase chain reaction (qRT-PCR), and CD31 and kinase insert 
      domain receptor (KDR), determined by immunofluorescence. Moreover, MIAMI cells 
      formed vascular endothelial-like tubules in the presence of matrigel. Bioplex 
      immunoassay analysis showed increased secretion of angiogenic/anti-inflammatory 
      factors by the MIAMI cells under 3% O(2) compared with 21% O(2), including monocyte 
      chemoattractant protein-1 (MCP-1), fractalkine (Ftk), growth-related oncogene 
      (GRO), vascular endothelial growth factor (VEGF), interleukin (IL)-6 and IL-8. 
      Furthermore, transcripts for anti-inflammatory molecules stanniocalcin-1 (STC-1) 
      and tumor necrosis factor-alpha-stimulated gene 6 (TSG-6) were up-regulated several 
      fold. CONCLUSIONS: MIAMI cells can be very useful for patients affected by CLI. 
      MIAMI cells promote blood vessel formation and reduce inflammation and necrosis 
      in ischemic tissue.
FAU - Rahnemai-Azar, Amirali
AU  - Rahnemai-Azar A
AD  - Department of Surgery, University of Miami Miller School of Medicine, Miami, 
      Florida, USA.
FAU - D'Ippolito, Gianluca
AU  - D'Ippolito G
FAU - Gomez, Lourdes A
AU  - Gomez LA
FAU - Reiner, Teresita
AU  - Reiner T
FAU - Vazquez-Padron, Roberto I
AU  - Vazquez-Padron RI
FAU - Perez-Stable, Carlos
AU  - Perez-Stable C
FAU - Roos, Bernard A
AU  - Roos BA
FAU - Pham, Si M
AU  - Pham SM
FAU - Schiller, Paul C
AU  - Schiller PC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20100915
PL  - England
TA  - Cytotherapy
JT  - Cytotherapy
JID - 100895309
RN  - 0 (Angiogenic Proteins)
RN  - 0 (Cytokines)
RN  - 0 (Platelet Endothelial Cell Adhesion Molecule-1)
RN  - 0 (von Willebrand Factor)
RN  - EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-2)
SB  - IM
MH  - Adult Stem Cells/*physiology/*transplantation
MH  - Angiogenic Proteins/metabolism
MH  - Animals
MH  - Bone Marrow Cells
MH  - Cell Differentiation
MH  - Cytokines/metabolism
MH  - Fluorescent Antibody Technique
MH  - Hindlimb/*blood supply/injuries
MH  - Humans
MH  - Inflammation/therapy
MH  - Ischemia/*therapy
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Necrosis
MH  - *Neovascularization, Physiologic
MH  - Peripheral Vascular Diseases/*therapy
MH  - Platelet Endothelial Cell Adhesion Molecule-1/genetics/metabolism
MH  - Regional Blood Flow
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stem Cell Transplantation
MH  - Vascular Endothelial Growth Factor Receptor-2/genetics/metabolism
MH  - von Willebrand Factor/genetics/metabolism
EDAT- 2010/09/16 06:00
MHDA- 2011/07/27 06:00
CRDT- 2010/09/16 06:00
PHST- 2010/09/16 06:00 [entrez]
PHST- 2010/09/16 06:00 [pubmed]
PHST- 2011/07/27 06:00 [medline]
AID - S1465-3249(11)70494-7 [pii]
AID - 10.3109/14653249.2010.515579 [doi]
PST - ppublish
SO  - Cytotherapy. 2011 Feb;13(2):179-92. doi: 10.3109/14653249.2010.515579. Epub 2010 
      Sep 15.