PMID- 20840797
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20211020
IS  - 1755-8166 (Electronic)
IS  - 1755-8166 (Linking)
VI  - 3
DP  - 2010 Sep 15
TI  - Fluorescence in situ hybridization in combination with the comet assay and 
      micronucleus test in genetic toxicology.
PG  - 17
LID - 10.1186/1755-8166-3-17 [doi]
AB  - Comet assay and micronucleus (MN) test are widely applied in genotoxicity testing 
      and biomonitoring. While comet assay permits to measure direct DNA-strand 
      breaking capacity of a tested agent MN test allows estimating the induced amount 
      of chromosome and/or genome mutations. The potential of these two methods can be 
      enhanced by the combination with fluorescence in situ hybridization (FISH) 
      techniques. FISH plus comet assay allows the recognition of targets of DNA damage 
      and repairing directly. FISH combined with MN test is able to characterize the 
      occurrence of different chromosomes in MN and to identify potential chromosomal 
      targets of mutagenic substances. Thus, combination of FISH with the comet assay 
      or MN test proved to be promising techniques for evaluation of the distribution 
      of DNA and chromosome damage in the entire genome of individual cells. FISH 
      technique also permits to study comet and MN formation, necessary for correct 
      application of these methods. This paper reviews the relevant literature on 
      advantages and limitations of Comet-FISH and MN-FISH assays application in 
      genetic toxicology.
FAU - Hovhannisyan, Galina G
AU  - Hovhannisyan GG
AD  - Department of Genetics and Cytology, State University, Biological Faculty, 1 Alex 
      Manoukian Street, Yerevan 375025, Armenia. hovgalina@list.ru.
LA  - eng
PT  - Journal Article
DEP - 20100915
PL  - England
TA  - Mol Cytogenet
JT  - Molecular cytogenetics
JID - 101317942
PMC - PMC2949878
EDAT- 2010/09/16 06:00
MHDA- 2010/09/16 06:01
PMCR- 2010/09/15
CRDT- 2010/09/16 06:00
PHST- 2010/06/22 00:00 [received]
PHST- 2010/09/15 00:00 [accepted]
PHST- 2010/09/16 06:00 [entrez]
PHST- 2010/09/16 06:00 [pubmed]
PHST- 2010/09/16 06:01 [medline]
PHST- 2010/09/15 00:00 [pmc-release]
AID - 1755-8166-3-17 [pii]
AID - 10.1186/1755-8166-3-17 [doi]
PST - epublish
SO  - Mol Cytogenet. 2010 Sep 15;3:17. doi: 10.1186/1755-8166-3-17.