PMID- 20841567
OWN - NLM
STAT- MEDLINE
DCOM- 20110110
LR  - 20160513
IS  - 1937-9145 (Electronic)
IS  - 1945-0877 (Linking)
VI  - 3
IP  - 139
DP  - 2010 Sep 14
TI  - Characterization of the extrinsic apoptotic pathway in the basal chordate 
      amphioxus.
PG  - ra66
LID - 10.1126/scisignal.2000906 [doi]
AB  - The death receptor (DR)-mediated apoptosis pathway is thought to be unique to 
      vertebrates. However, the presence of DR-encoding genes in the sea urchin and the 
      basal chordate amphioxus prompted us to reconsider, especially given that 
      amphioxus contains 14 DR proteins and hundreds of death domain (DD)-containing 
      adaptor proteins. To understand how the extrinsic apoptotic pathway was 
      originally established and what the differences in signaling are between 
      invertebrates and vertebrates, we performed functional studies of several genes 
      that encode DDs in the amphioxus Branchiostoma belcheri tsingtauense (Bbt). 
      First, we observed that the increased abundance of Bbt Fas-associated death 
      domain 1 (BbtFADD1) in HeLa cells resulted in the formation of death effector 
      filamentous structures in the cytoplasm and the activation of the nuclear factor 
      kappaB pathway, whereas BbtFADD2 protein was restricted to the nucleus, although its 
      death effector domain induced apoptosis when in the cytoplasm. We further 
      demonstrated that formation of a FADD-caspase-8 complex recruited amphioxus DR1 
      (BbtDR1), which bound to the adaptor proteins CRADD or TRAF6 (tumor necrosis 
      factor receptor-associated factor 6) to convey distinct signals, ranging from 
      apoptosis to gene activation. Thus, our study not only reveals the evolutionary 
      origin of the extrinsic apoptotic pathway in a basal chordate but also adds to 
      our understanding of the similarities and differences between invertebrate and 
      vertebrate FADD signaling.
FAU - Yuan, Shaochun
AU  - Yuan S
AD  - State Key Laboratory of Biocontrol, National Engineering Research Center of South 
      China Sea Marine Biotechnology, Department of Biochemistry, College of Life 
      Sciences, Sun Yat-sen University, 135 West Xingang Road, Guangzhou 510275, P. R. 
      China.
FAU - Liu, Huiling
AU  - Liu H
FAU - Gu, Ming
AU  - Gu M
FAU - Xu, Liqun
AU  - Xu L
FAU - Huang, Shengfeng
AU  - Huang S
FAU - Ren, Zhenhua
AU  - Ren Z
FAU - Xu, Anlong
AU  - Xu A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100914
PL  - United States
TA  - Sci Signal
JT  - Science signaling
JID - 101465400
RN  - 0 (Fas-Associated Death Domain Protein)
RN  - 0 (NF-kappa B)
RN  - 0 (TNF Receptor-Associated Factor 6)
RN  - EC 3.4.22.- (Caspase 8)
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Caspase 8/genetics/*metabolism
MH  - Chordata, Nonvertebrate/embryology/genetics/*metabolism
MH  - Fas-Associated Death Domain Protein/genetics/*metabolism
MH  - HEK293 Cells
MH  - HeLa Cells
MH  - Humans
MH  - NF-kappa B/genetics/*metabolism
MH  - Protein Structure, Tertiary
MH  - Signal Transduction/physiology
MH  - TNF Receptor-Associated Factor 6/genetics/*metabolism
EDAT- 2010/09/16 06:00
MHDA- 2011/01/11 06:00
CRDT- 2010/09/16 06:00
PHST- 2010/09/16 06:00 [entrez]
PHST- 2010/09/16 06:00 [pubmed]
PHST- 2011/01/11 06:00 [medline]
AID - 3/139/ra66 [pii]
AID - 10.1126/scisignal.2000906 [doi]
PST - epublish
SO  - Sci Signal. 2010 Sep 14;3(139):ra66. doi: 10.1126/scisignal.2000906.