PMID- 20842468
OWN - NLM
STAT- MEDLINE
DCOM- 20110321
LR  - 20211020
IS  - 1937-5395 (Electronic)
IS  - 1937-5387 (Print)
IS  - 1937-5387 (Linking)
VI  - 3
IP  - 6
DP  - 2010 Dec
TI  - Assessment of metabolic phenotypes in patients with non-ischemic dilated 
      cardiomyopathy undergoing cardiac resynchronization therapy.
PG  - 643-51
LID - 10.1007/s12265-010-9223-5 [doi]
AB  - Studies of myocardial metabolism have reported that contractile performance at a 
      given myocardial oxygen consumption (MVO2) can be lower when the heart is 
      oxidizing fatty acids rather than glucose or lactate. The objective of this study 
      is to assess the prognostic value of myocardial metabolic phenotypes in 
      identifying non-responders among non-ischemic dilated cardiomyopathy (NIDCM) 
      patients undergoing cardiac resynchronization therapy (CRT). Arterial and 
      coronary sinus plasma concentrations of oxygen, glucose, lactate, pyruvate, free 
      fatty acids (FFA), and 22 amino acids were obtained from 19 male and 2 female 
      patients (mean age 56 +/- 16) with NIDCM undergoing CRT. Metabolite fluxes/MVO2 and 
      extraction fractions were calculated. Flux balance analysis (FBA) was performed 
      with MetaFluxNet 1.8 on a metabolic network of the cardiac mitochondria (189 
      reactions, 230 metabolites) reconstructed from mitochondrial proteomic data (615 
      proteins) from human heart tissue. Non-responders based on left ventricular 
      ejection fraction (LVEF) demonstrated a greater mean FFA extraction fraction 
      (35% +/- 17%) than responders [18 +/- 10%, p = 0.0098, area under the estimated ROC 
      curve (AUC) was 0.8238, S.E. 0.1115]. Calculated adenosine triphosphate 
      (ATP)/MVO2 using FBA correlated with change in New York Heart Association (NYHA) 
      class (rho = 0.63, p = 0.0298; AUC = 0.8381, S.E. 0.1316). Non-responders based 
      on both LVEF and NYHA demonstrated a greater mean FFA uptake/MVO2 (0.115 +/- 0.112) 
      than responders (0.034 +/- 0.030, p = 0.0171; AUC = 0.8593, S.E. 0.0965). 
      Myocardial FFA flux and calculated maximal ATP synthesis flux using FBA may be 
      helpful as biomarkers in identifying non-responders among NIDCM patients 
      undergoing CRT.
FAU - Obrzut, Sebastian
AU  - Obrzut S
AD  - Division of Nuclear Medicine, University of California-San Diego, UCSD Medical 
      Center-Hillcrest, 200 West Arbor Drive, San Diego, CA 92103-8758, USA. 
      sobrzut@ucsd.edu
FAU - Tiongson, Jay
AU  - Tiongson J
FAU - Jamshidi, Neema
AU  - Jamshidi N
FAU - Phan, Huy Minh
AU  - Phan HM
FAU - Hoh, Carl
AU  - Hoh C
FAU - Birgersdotter-Green, Ulrika
AU  - Birgersdotter-Green U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100915
PL  - United States
TA  - J Cardiovasc Transl Res
JT  - Journal of cardiovascular translational research
JID - 101468585
RN  - 0 (Amino Acids)
RN  - 0 (Biomarkers)
RN  - 0 (Blood Glucose)
RN  - 0 (Fatty Acids, Nonesterified)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 8558G7RUTR (Pyruvic Acid)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Adult
MH  - Aged
MH  - Amino Acids/blood
MH  - Biomarkers/blood
MH  - Blood Glucose/metabolism
MH  - *Cardiac Resynchronization Therapy
MH  - Cardiomyopathy, Dilated/blood/physiopathology/*therapy
MH  - *Energy Metabolism
MH  - Fatty Acids, Nonesterified/blood
MH  - Female
MH  - Heart Failure/blood/physiopathology/*therapy
MH  - Humans
MH  - Lactic Acid/blood
MH  - Male
MH  - Middle Aged
MH  - Mitochondria, Heart/metabolism
MH  - Myocardial Contraction
MH  - Myocardium/*metabolism
MH  - Oxygen/blood
MH  - *Oxygen Consumption
MH  - Phenotype
MH  - Prospective Studies
MH  - Proteomics
MH  - Pyruvic Acid/blood
MH  - Stroke Volume
MH  - Time Factors
MH  - Treatment Failure
MH  - *Ventricular Function, Left
PMC - PMC2993897
EDAT- 2010/09/16 06:00
MHDA- 2011/03/22 06:00
PMCR- 2010/09/15
CRDT- 2010/09/16 06:00
PHST- 2010/05/27 00:00 [received]
PHST- 2010/09/01 00:00 [accepted]
PHST- 2010/09/16 06:00 [entrez]
PHST- 2010/09/16 06:00 [pubmed]
PHST- 2011/03/22 06:00 [medline]
PHST- 2010/09/15 00:00 [pmc-release]
AID - 9223 [pii]
AID - 10.1007/s12265-010-9223-5 [doi]
PST - ppublish
SO  - J Cardiovasc Transl Res. 2010 Dec;3(6):643-51. doi: 10.1007/s12265-010-9223-5. 
      Epub 2010 Sep 15.