PMID- 20842640
OWN - NLM
STAT- MEDLINE
DCOM- 20110901
LR  - 20131121
IS  - 1099-1352 (Electronic)
IS  - 0952-3499 (Linking)
VI  - 24
IP  - 4
DP  - 2011 Jul-Aug
TI  - Diversity of DNA-hydrolyzing antibodies from the sera of autoimmune-prone 
      MRL/MpJ-lpr mice.
PG  - 557-69
LID - 10.1002/jmr.1067 [doi]
AB  - It has been shown for the first time that polyclonal IgG abzymes (Abzs) with 
      DNase activity from the sera of autoimmune-prone MRL/MpJ-lpr mice can be 
      separated by isoelectric focusing into many subfractions having the isoelectric 
      points (pI) from 4.5 to 9, with the maximal activity for Abzs with pI = 6.5-9.0. 
      Affinity chromatography on DNA-cellulose separated DNase IgGs into many 
      subfractions demonstrating a range of affinities for DNA and different levels of 
      the relative DNase activities (RDA) due to intrinsically bound metals and after 
      addition of external Mg(2+) , Mn(2+) , Ca(2+), and Mg(2+) +Ca(2+). Some fractions 
      significantly increase RDAs in the presence of external ions (Mg(2+) + Ca(2+)  > 
      Mg(2+)  > Mn(2+)  > Ca(2+)), while each of this cofactor can also inhibit or have 
      no influence on the RDAs of another fractions. It is known that complexes of DNA 
      with histones and other proteins of apoptotic cells are the primary immunogens in 
      systemic lupus erythematosus (SLE). Bovine serum albumin (BSA) and methylated BSA 
      (mBSA) increase the RDAs of only some fractions, while have no effect or inhibit 
      other IgG fractions. The ratio of the RDAs in the presence of all metal ions, 
      BSA, and mBSA was individual for every abzyme fraction. Mn(2+) and Ca(2+) 
      stimulated accumulation of only relaxed form of supercoiled DNA (scDNA) in the 
      case of all subfractions, while in the presence of Mg(2+) antibodies (Abs) of 
      some subfractions (and in the presence of Mn(2+) +Ca(2+) all subfractions) 
      produced relaxed DNA (rDNA) and linear DNA (linDNA) in a variable extent. The 
      data obtained show that the polyclonal Abzs of mice may be a cocktail of Abs 
      directly to DNA, RNA, and their complexes with proteins and anti-idiotypic Abs to 
      active centers of different nucleases. The diversity of the physicochemical and 
      kinetic characteristics of the Abzs seems to be significantly widened when 
      pre-diseased mice spontaneously develop the disease.
CI  - Copyright (c) 2010 John Wiley & Sons, Ltd.
FAU - Kostrikina, Irina A
AU  - Kostrikina IA
AD  - Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the 
      Russian Academy of Sciences, pr. Lavrent'eva 8, Novosibirsk 630090, Russia.
FAU - Kolesova, Marina E
AU  - Kolesova ME
FAU - Orlovskaya, Irina A
AU  - Orlovskaya IA
FAU - Buneva, Valentina N
AU  - Buneva VN
FAU - Nevinsky, Georgy A
AU  - Nevinsky GA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100914
PL  - England
TA  - J Mol Recognit
JT  - Journal of molecular recognition : JMR
JID - 9004580
RN  - 0 (Antibodies, Catalytic)
RN  - 0 (DNA-cellulose)
RN  - 42Z2K6ZL8P (Manganese)
RN  - 9004-34-6 (Cellulose)
RN  - 9007-49-2 (DNA)
RN  - EC 3.1.- (Deoxyribonucleases)
RN  - I38ZP9992A (Magnesium)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Antibodies, Catalytic/*metabolism
MH  - Calcium/pharmacology
MH  - Cattle
MH  - Cellulose/analogs & derivatives/chemistry
MH  - Chromatography, Affinity
MH  - DNA/chemistry/*metabolism
MH  - Deoxyribonucleases/*metabolism
MH  - Enzyme Activation/drug effects
MH  - Isoelectric Focusing
MH  - Magnesium/pharmacology
MH  - Manganese/pharmacology
MH  - Mice
MH  - Mice, Inbred MRL lpr
EDAT- 2010/09/16 06:00
MHDA- 2011/09/02 06:00
CRDT- 2010/09/16 06:00
PHST- 2010/05/12 00:00 [received]
PHST- 2010/06/16 00:00 [revised]
PHST- 2010/06/17 00:00 [accepted]
PHST- 2010/09/16 06:00 [entrez]
PHST- 2010/09/16 06:00 [pubmed]
PHST- 2011/09/02 06:00 [medline]
AID - 10.1002/jmr.1067 [doi]
PST - ppublish
SO  - J Mol Recognit. 2011 Jul-Aug;24(4):557-69. doi: 10.1002/jmr.1067. Epub 2010 Sep 
      14.