PMID- 20842647
OWN - NLM
STAT- MEDLINE
DCOM- 20110322
LR  - 20240316
IS  - 1542-0760 (Electronic)
IS  - 1542-0752 (Print)
IS  - 1542-0752 (Linking)
VI  - 88
IP  - 11
DP  - 2010 Nov
TI  - Magnetic resonance microscopy-based analyses of the brains of normal and 
      ethanol-exposed fetal mice.
PG  - 953-64
LID - 10.1002/bdra.20719 [doi]
AB  - BACKGROUND: The application of magnetic resonance microscopy (MRM) to the study 
      of normal and abnormal prenatal mouse development has facilitated discovery of 
      dysmorphology following prenatal ethanol insult. The current analyses extend this 
      work, providing a regional brain volume-based description of normal brain growth 
      and illustrating the consequences of gestational day (GD) 10 ethanol exposure in 
      the fetal mouse. METHODS: To assess normal growth, control C57Bl/6J fetuses 
      collected on GD 16, GD 16.5, and GD 17 were scanned using a 9.4-T magnet, 
      resulting in 29-mum isotropic resolution images. For the ethanol teratogenicity 
      studies, C57Bl/6J dams were administered intraperitoneal ethanol (2.9 g/kg) at 10 
      days, 0 hr, and 10 days, 4 hr, after fertilization, and fetuses were collected 
      for analyses on GD 17. From individual MRM scans, linear measurements and 
      regional brain volumes were determined and compared. RESULTS: In control fetuses, 
      each of the assessed brain regions increased in volume, whereas ventricular 
      volumes decreased between GD 16 and GD 17. Illustrating a global developmental 
      delay, prenatal ethanol exposure resulted in reduced body volumes, crown-rump 
      lengths, and a generalized decrease in regional brain volumes compared with GD 17 
      controls. However, compared with GD 16.5, morphologically matched controls, 
      ethanol exposure resulted in volume increases in the lateral and third ventricles 
      as well as a disproportionate reduction in cortical volume. CONCLUSIONS: The 
      normative data collected in this study facilitate the distinction between GD 10 
      ethanol-induced developmental delay and frank dysmorphology. This work 
      illustrates the utility of MRM-based analyses for developmental toxicology 
      studies and extends our knowledge of the stage-dependency of ethanol 
      teratogenesis.
CI  - Copyright (c) 2010 Wiley-Liss, Inc.
FAU - O'Leary-Moore, Shonagh K
AU  - O'Leary-Moore SK
AD  - Fetal Toxicology Division,Bowles Center for Alcohol Studies, University of North 
      Carolina at Chapel Hill,Chapel Hill, NC 27599-7178E, USA. somoore@med.unc.edu
FAU - Parnell, Scott E
AU  - Parnell SE
FAU - Godin, Elizabeth A
AU  - Godin EA
FAU - Dehart, Deborah B
AU  - Dehart DB
FAU - Ament, Jacob J
AU  - Ament JJ
FAU - Khan, Amber A
AU  - Khan AA
FAU - Johnson, G Allan
AU  - Johnson GA
FAU - Styner, Martin A
AU  - Styner MA
FAU - Sulik, Kathleen K
AU  - Sulik KK
LA  - eng
GR  - HD 03110/HD/NICHD NIH HHS/United States
GR  - P41 RR005959/RR/NCRR NIH HHS/United States
GR  - P60 AA011605/AA/NIAAA NIH HHS/United States
GR  - P50 AA011605/AA/NIAAA NIH HHS/United States
GR  - P41 05959/PHS HHS/United States
GR  - P30 HD003110/HD/NICHD NIH HHS/United States
GR  - R00 AA018697/AA/NIAAA NIH HHS/United States
GR  - AA007573/AA/NIAAA NIH HHS/United States
GR  - U24 CA092656/CA/NCI NIH HHS/United States
GR  - U01 AA017124/AA/NIAAA NIH HHS/United States
GR  - T32 AA007573/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20100914
PL  - United States
TA  - Birth Defects Res A Clin Mol Teratol
JT  - Birth defects research. Part A, Clinical and molecular teratology
JID - 101155107
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Abnormalities, Drug-Induced/pathology
MH  - Animals
MH  - Brain/abnormalities/*drug effects/embryology
MH  - Disease Models, Animal
MH  - Ethanol/*toxicity
MH  - Female
MH  - Fetal Alcohol Spectrum Disorders/pathology
MH  - Fetus/*drug effects/pathology
MH  - Gestational Age
MH  - Humans
MH  - Magnetic Resonance Imaging/*methods
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy/methods
MH  - Pregnancy
MH  - Prenatal Exposure Delayed Effects/chemically induced/pathology
PMC - PMC3445267
MID - NIHMS396785
EDAT- 2010/09/16 06:00
MHDA- 2011/03/23 06:00
PMCR- 2012/09/18
CRDT- 2010/09/16 06:00
PHST- 2010/06/01 00:00 [received]
PHST- 2010/06/23 00:00 [revised]
PHST- 2010/07/02 00:00 [accepted]
PHST- 2010/09/16 06:00 [entrez]
PHST- 2010/09/16 06:00 [pubmed]
PHST- 2011/03/23 06:00 [medline]
PHST- 2012/09/18 00:00 [pmc-release]
AID - 10.1002/bdra.20719 [doi]
PST - ppublish
SO  - Birth Defects Res A Clin Mol Teratol. 2010 Nov;88(11):953-64. doi: 
      10.1002/bdra.20719. Epub 2010 Sep 14.