PMID- 20842848
OWN - NLM
STAT- MEDLINE
DCOM- 20120124
LR  - 20161125
IS  - 1001-5515 (Print)
IS  - 1001-5515 (Linking)
VI  - 27
IP  - 4
DP  - 2010 Aug
TI  - [Electrical stimulation enhances viability of osteoblasts on conductive 
      biodegradable material].
PG  - 801-5
AB  - This study was aimed at exploring the viability of osteoblasts on conductive 
      tissue engineering material. Conductive biodegradable Polyprrole/Polylactide 
      (PPy/PLA) was prepared by emulsion polymerization. Scanning electron microscope 
      (SEM) and spectroscopy showed evenly dispersed PPy in PLA. PPy/PLA membrane was 
      found being able to keep conductive stability for more than one month to provide 
      electric circumstances (ECs) for osteoblasts. SEM displayed that osteoblasts 
      adhered and spread well on PPy/PLA. ECs of 12.5, 25, 50, 75, 100, 125, 150, 175, 
      200 microA/cm2 were separately used to stimulate osteoblasts for 24h, 48h, 72h 
      and 96h. Methyl-thiazole-tetrazolium (MTT) assay after 24h revealed that 50 
      microA/cm2 evidently accelerated osteoblasts proliferation. Alkaline phosphatase 
      (ALP) activity assay revealed that, 48h later, 50 microA/cm2, and 75 microA/cm2 
      promoted osteoblasts differentiation. 50 microA/cm2 enhanced osteoblasts 
      mineration. Conclusively, 50 microA/cm2 can strengthen osteoblasts' function and 
      promote their viability.
FAU - Men, Jiying
AU  - Men J
AD  - School of Chemical Engineering and Environment, Northern University of China, 
      Taiyuan 030051, China. menjiying@yahoo.com.cn
FAU - Deng, Xiaoyan
AU  - Deng X
FAU - Xu, Zaipin
AU  - Xu Z
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
JT  - Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = 
      Shengwu yixue gongchengxue zazhi
JID - 9426398
RN  - 0 (Biocompatible Materials)
RN  - 0 (Polyesters)
RN  - 0 (Polymers)
RN  - 0 (Pyrroles)
RN  - 30604-81-0 (polypyrrole)
RN  - 459TN2L5F5 (poly(lactide))
SB  - IM
MH  - *Absorbable Implants
MH  - Biocompatible Materials/chemistry/metabolism
MH  - Cell Differentiation
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Electric Conductivity
MH  - *Electric Stimulation
MH  - Humans
MH  - Osteoblasts/*cytology
MH  - Polyesters/*chemistry
MH  - Polymers/*chemistry
MH  - Pyrroles/*chemistry
MH  - Tissue Scaffolds/chemistry
EDAT- 2010/09/17 06:00
MHDA- 2012/01/25 06:00
CRDT- 2010/09/17 06:00
PHST- 2010/09/17 06:00 [entrez]
PHST- 2010/09/17 06:00 [pubmed]
PHST- 2012/01/25 06:00 [medline]
PST - ppublish
SO  - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2010 Aug;27(4):801-5.