PMID- 20844007
OWN - NLM
STAT- MEDLINE
DCOM- 20101104
LR  - 20211020
IS  - 1945-7170 (Electronic)
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 151
IP  - 11
DP  - 2010 Nov
TI  - Deficiency of scavenger receptor class B type I negatively affects progesterone 
      secretion in human granulosa cells.
PG  - 5519-27
LID - 10.1210/en.2010-0347 [doi]
AB  - Our goal was to examine the effect of deficiency of the lipoprotein receptor, 
      scavenger receptor class B type I (SR-BI), on progesterone secretion in human 
      granulosa cells (HGL5). Scrambled or SR-BI small interfering RNA [knockdown (KD)] 
      cells were exposed to dimethylsulfoxide [DMSO, vehicle for forskolin (Fo)], Fo, 
      serum, high-density lipoprotein, low-density lipoprotein (LDL), or Fo plus 
      lipoproteins or serum for 24 h. Progesterone secretion was lower in all of the 
      SR-BI KD cells regardless of treatment. We examined progesterone secretion in 
      SR-BI KD, LDL receptor KD, and double KD cells incubated with DMSO, Fo, LDL, or 
      Fo + LDL for 6-24 h. As compared with scrambled cells, progesterone secretion was 
      lower in SR-BI and double KD cells regardless of treatment; whereas progesterone 
      secretion was only lower in LDL receptor KD cells incubated with LDL and Fo + 
      LDL. We measured phosphorylation of hormone-sensitive lipase (pHSL) expression, 
      intracellular total cholesterol (TC) mass, and progesterone secretion in 
      scrambled and SR-BI KD cells incubated with DMSO or Fo for 2-24 h. The expression 
      of pHSL was similar between the cells and conditions. The mean change in TC mass 
      and progesterone secretion was lower in SR-BI KD cells exposed to DMSO and Fo. 
      Incubating SR-BI KD cells with 22-hydroxy cholesterol did not overcome the 
      reduction in progesterone secretion. At different time points, RNA expression of 
      steroidogenic acute regulatory protein, side-chain cleavage, and 
      3beta-hydroxysteroid dehydrogenase was significantly lower in SR-BI KD cells 
      incubated with Fo. In conclusion, SR-BI protein deficiency, in part, might 
      explain progesterone deficiency in some infertile women.
FAU - Kolmakova, Antonina
AU  - Kolmakova A
AD  - Department of Medicine, Division of Endocrinology, Johns Hopkins University 
      School of Medicine, Baltimore, Maryland 21215, USA.
FAU - Wang, Jiangxia
AU  - Wang J
FAU - Brogan, Rebecca
AU  - Brogan R
FAU - Chaffin, Charles
AU  - Chaffin C
FAU - Rodriguez, Annabelle
AU  - Rodriguez A
LA  - eng
GR  - P30 DK079637/DK/NIDDK NIH HHS/United States
GR  - R01 HD043358/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100915
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Lipoproteins, HDL)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Receptors, LDL)
RN  - 0 (SCARB1 protein, human)
RN  - 0 (Scavenger Receptors, Class B)
RN  - 4G7DS2Q64Y (Progesterone)
SB  - IM
MH  - Blotting, Western
MH  - Cell Line
MH  - Cells, Cultured
MH  - Female
MH  - Granulosa Cells/drug effects/*metabolism
MH  - Humans
MH  - Lipoproteins, HDL/metabolism/pharmacology
MH  - Lipoproteins, LDL/metabolism/pharmacology
MH  - Progesterone/*metabolism
MH  - RNA, Small Interfering
MH  - Radioimmunoassay
MH  - Receptors, LDL/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Scavenger Receptors, Class B/genetics/*metabolism
PMC - PMC3208332
EDAT- 2010/09/17 06:00
MHDA- 2010/11/05 06:00
PMCR- 2011/11/01
CRDT- 2010/09/17 06:00
PHST- 2010/09/17 06:00 [entrez]
PHST- 2010/09/17 06:00 [pubmed]
PHST- 2010/11/05 06:00 [medline]
PHST- 2011/11/01 00:00 [pmc-release]
AID - en.2010-0347 [pii]
AID - 5631 [pii]
AID - 10.1210/en.2010-0347 [doi]
PST - ppublish
SO  - Endocrinology. 2010 Nov;151(11):5519-27. doi: 10.1210/en.2010-0347. Epub 2010 Sep 
      15.