PMID- 20845999
OWN - NLM
STAT- MEDLINE
DCOM- 20101216
LR  - 20191111
IS  - 0028-2685 (Print)
IS  - 0028-2685 (Linking)
VI  - 57
IP  - 6
DP  - 2010
TI  - Prevalence of high-risk human papillomavirus types (HPV-16, HPV-18) and their 
      physical status in primary laryngeal squamous cell carcinoma.
PG  - 594-600
AB  - Recently oncogenetic human papilloma virus(HPV) infection has been suggested to 
      promote laryngeal squamous cell carcinoma(LSCC). To determine the prevalence and 
      genotypes of HPV infection in laryngeal cancer specimens, 84 specimens from 
      pathologically confirmed LSCC patients were studied for the presence of viral DNA 
      and possible virus integration into the cellular genome. HPV genotyping was 
      assayed prior to the integration analysis by using two PCR-based assays, 
      including HPV-16 and-18 E7 type-specific and L1 general primers (GP5+/GP6+). 
      Additionally, a quantitative real-time PCR (qRT-PCR) was used to examine the 
      physical status of HPV-16 or-18 in HPV positive LSCC. Using HPV L1 general primer 
      amplification, HPV DNA was detected in 23 (27.4%) of the 84 LSCC samples. When 
      PCR products were cloned and sequenced, HPV16 were found in all 23 L1 positive 
      samples. However, when specific primers for HPV- 16 or -18 were used to amplify 
      E6 and E7 in all samples, 29 cases (34.5%) were positive for HPV-16, while 6 
      cases (7.1%) were positive for HPV 18. Coinfection of HPV-16 and -18 were found 
      in 4 cases (4.8%). In addition, qRT-PCR assay found that HPV-16 was characterized 
      as episomal in 51.7% of cases, mixed (i.e., episomal and integrated) in 34.5%, 
      and integrated in 13.8%, while HPV-18 was similarly characterized as episomal in 
      83.3% of cases and mixed in 16.7%. In the present study, about 36.9% of patients 
      with LSCC were found to be infected with HPV-16 and -18 and integration of HPV-16 
      and -18 DNA into the host genome was found.
FAU - Liu, B
AU  - Liu B
AD  - Department of Genetics, Peking University School of Oncology, Beijing, China. 
      lbg29@sohu.com
FAU - Lu, Z
AU  - Lu Z
FAU - Wang, P
AU  - Wang P
FAU - Basang, Z
AU  - Basang Z
FAU - Rao, X
AU  - Rao X
LA  - eng
PT  - Journal Article
PL  - Slovakia
TA  - Neoplasma
JT  - Neoplasma
JID - 0377266
RN  - 0 (Capsid Proteins)
RN  - 0 (DNA, Viral)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (E7 protein, Human papillomavirus type 18)
RN  - 0 (Oncogene Proteins, Viral)
RN  - 0 (Papillomavirus E7 Proteins)
RN  - 0 (oncogene protein E7, Human papillomavirus type 16)
RN  - 6LTE2DNX63 (L1 protein, Human papillomavirus type 16)
RN  - J2D279PEM5 (L1 protein, Human papillomavirus type 18)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Capsid Proteins/genetics
MH  - Carcinoma, Squamous Cell/*virology
MH  - DNA, Viral/analysis
MH  - DNA-Binding Proteins/genetics
MH  - Female
MH  - Human papillomavirus 16/genetics/*isolation & purification
MH  - Human papillomavirus 18/genetics/*isolation & purification
MH  - Humans
MH  - Laryngeal Neoplasms/*virology
MH  - Male
MH  - Middle Aged
MH  - Oncogene Proteins, Viral/genetics
MH  - Papillomavirus E7 Proteins/genetics
MH  - Polymerase Chain Reaction
EDAT- 2010/09/18 06:00
MHDA- 2010/12/17 06:00
CRDT- 2010/09/18 06:00
PHST- 2010/09/18 06:00 [entrez]
PHST- 2010/09/18 06:00 [pubmed]
PHST- 2010/12/17 06:00 [medline]
AID - 10.4149/neo_2010_06_594 [doi]
PST - ppublish
SO  - Neoplasma. 2010;57(6):594-600. doi: 10.4149/neo_2010_06_594.