PMID- 20846345
OWN - NLM
STAT- MEDLINE
DCOM- 20110211
LR  - 20210102
IS  - 1478-3231 (Electronic)
IS  - 1478-3223 (Linking)
VI  - 30
IP  - 10
DP  - 2010 Nov
TI  - Fibrogenic cell phenotype modifications during remodelling of normal and 
      pathological human liver in cultured slices.
PG  - 1529-40
LID - 10.1111/j.1478-3231.2010.02342.x [doi]
AB  - BACKGROUND: The debate concerning the potential remodelling and/or reversibility 
      of cirrhotic lesions and biliary fibrosis is still open. AIMS/METHODS: In this 
      work, we have used the precision-cut liver slice (PCLS) model, which maintains 
      cell-cell and cell-matrix interactions to study, by immunohistochemistry, the 
      behaviour of the different fibrogenic cells, i.e. hepatic stellate cells (HSC) 
      and portal fibroblasts, in cultured (for 1 week) PCLS derived from normal and 
      fibrotic human livers. RESULTS: In normal liver, before and after culture, 
      alpha-smooth muscle (SM) actin was present only in the vessel walls. Platelet-derived 
      growth factor (PDGF) receptor-beta was expressed before and after culture by portal 
      fibroblasts, and appeared after culture in HSC. Before culture, CD 34 was not 
      expressed in parenchyma, but appeared after culture in sinusoidal endothelial 
      cells. In cirrhotic lesions, before culture, alpha-SM actin, PDGF receptor-beta and 
      Thy-1 were expressed in septa; after culture, alpha-SM actin expression disappeared 
      but the expression of the PDGF receptor-beta and Thy-1 was maintained. In 
      cholestatic liver specimens, alpha-SM actin, PDGF receptor-beta and Thy-1 expression, 
      which was present before culture in enlarged portal areas, disappeared after 
      culture, and apoptosis was detected. In the parenchyma of both cirrhotic and 
      cholestatic livers, the expression of the PDGF receptor-beta and of CD 34, which was 
      not observed before culture, was present in HSC and sinusoidal endothelial cells, 
      respectively, after culture. CONCLUSIONS: These results indicate that during 
      remodelling of pathological tissues in cultured liver slices, the myofibroblastic 
      cells derived from HSC or from portal fibroblasts show different behaviours, 
      suggesting different mechanisms of activation/deactivation.
CI  - (c) 2010 John Wiley & Sons A/S.
FAU - Guyot, Christelle
AU  - Guyot C
AD  - INSERM U889 and Universite Victor Segalen Bordeaux 2, Bordeaux, France.
FAU - Lepreux, Sebastien
AU  - Lepreux S
FAU - Combe, Chantal
AU  - Combe C
FAU - Sarrazy, Vincent
AU  - Sarrazy V
FAU - Billet, Fabrice
AU  - Billet F
FAU - Balabaud, Charles
AU  - Balabaud C
FAU - Bioulac-Sage, Paulette
AU  - Bioulac-Sage P
FAU - Desmouliere, Alexis
AU  - Desmouliere A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100916
PL  - United States
TA  - Liver Int
JT  - Liver international : official journal of the International Association for the 
      Study of the Liver
JID - 101160857
RN  - 0 (Actins)
RN  - 0 (Antigens, CD34)
RN  - 0 (Biomarkers)
RN  - 0 (Keratin-19)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Thy-1 Antigens)
RN  - EC 2.7.10.1 (Receptor, Platelet-Derived Growth Factor beta)
SB  - IM
MH  - Actins/metabolism
MH  - Aged
MH  - Antigens, CD34/metabolism
MH  - Apoptosis
MH  - Bile Ducts, Intrahepatic/metabolism/pathology
MH  - Biomarkers/metabolism
MH  - Cell Proliferation
MH  - *Cell Transdifferentiation
MH  - Cholestasis, Intrahepatic/metabolism/*pathology
MH  - Endothelial Cells/metabolism/pathology
MH  - Female
MH  - Fibroblasts/metabolism/*pathology
MH  - Hepatic Stellate Cells/metabolism/*pathology
MH  - Humans
MH  - Immunohistochemistry
MH  - Keratin-19/metabolism
MH  - Ki-67 Antigen/metabolism
MH  - Liver/metabolism/*pathology
MH  - Liver Cirrhosis/metabolism/*pathology
MH  - Male
MH  - Middle Aged
MH  - Myofibroblasts/metabolism/pathology
MH  - Phenotype
MH  - Receptor, Platelet-Derived Growth Factor beta/metabolism
MH  - Thy-1 Antigens/metabolism
MH  - Time Factors
MH  - Tissue Culture Techniques
EDAT- 2010/09/18 06:00
MHDA- 2011/02/12 06:00
CRDT- 2010/09/18 06:00
PHST- 2010/09/18 06:00 [entrez]
PHST- 2010/09/18 06:00 [pubmed]
PHST- 2011/02/12 06:00 [medline]
AID - 10.1111/j.1478-3231.2010.02342.x [doi]
PST - ppublish
SO  - Liver Int. 2010 Nov;30(10):1529-40. doi: 10.1111/j.1478-3231.2010.02342.x. Epub 
      2010 Sep 16.