PMID- 20846492
OWN - NLM
STAT- MEDLINE
DCOM- 20101228
LR  - 20100917
IS  - 1976-670X (Electronic)
IS  - 1976-6696 (Linking)
VI  - 43
IP  - 9
DP  - 2010 Sep
TI  - Inhibitory effects of antithrombin on the expression of secretory group IIA 
      phospholipase A(2) in endothelial cells.
PG  - 604-8
LID - 10.5483/BMBRep.2010.43.9.604 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) mediates proinflammatory responses in primary 
      human umbilical vein endothelial cells (HUVECs), and it upregulates the 
      expression of secretory group IIA phospholipase A(2) (sPLA(2)-IIA). sPLA(2)-IIA 
      plays a pivotal role in inflammation, and antithrombin (AT) possesses properties 
      that are beneficial to endothelial cells. Therefore, we investigated the effects 
      of AT on the expression of sPLA(2)-IIA in TNF-alpha-stimulated HUVECs. TNF-alpha potently 
      upregulated the expression of sPLA(2)-IIA, and prior treatment of cells with AT 
      inhibited the expression of sPLA(2)-IIA in HUVECs. Also, antibodies or siRNA for 
      syndecan-4 blocked the protective effect of AT. Furthermore, PI3-kinase and the 
      AKT pathway are significantly involved in the AT-mediated inhibition of the 
      expression of sPLA(2)-IIA. These results show that AT effectively suppresses the 
      upregulated sPLA(2)-IIA expression, which might contribute to the cytoprotective 
      effects of AT in the treatment of severe inflammatory diseases.
FAU - Kim, Tae Hoon
AU  - Kim TH
AD  - Department of Herbal Pharmaceutical Engineering, Daegu Haany University, 
      Gyeongsan, Korea.
FAU - Bae, Jong-Sup
AU  - Bae JS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - BMB Rep
JT  - BMB reports
JID - 101465334
RN  - 0 (Antithrombins)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (SDC4 protein, human)
RN  - 0 (Syndecan-4)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.1.1.4 (Group II Phospholipases A2)
SB  - IM
MH  - Antithrombins/*pharmacology
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Endothelial Cells/*drug effects/*enzymology
MH  - Enzyme Activation/drug effects/genetics
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Group II Phospholipases A2/*metabolism
MH  - Humans
MH  - Inhibitory Concentration 50
MH  - RNA, Small Interfering
MH  - Syndecan-4/genetics/metabolism
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2010/09/18 06:00
MHDA- 2010/12/29 06:00
CRDT- 2010/09/18 06:00
PHST- 2010/09/18 06:00 [entrez]
PHST- 2010/09/18 06:00 [pubmed]
PHST- 2010/12/29 06:00 [medline]
AID - 10.5483/BMBRep.2010.43.9.604 [doi]
PST - ppublish
SO  - BMB Rep. 2010 Sep;43(9):604-8. doi: 10.5483/BMBRep.2010.43.9.604.