PMID- 20847375
OWN - NLM
STAT- MEDLINE
DCOM- 20110124
LR  - 20220317
IS  - 0971-5916 (Print)
IS  - 0971-5916 (Linking)
VI  - 132
DP  - 2010 Sep
TI  - Assessment of HER-2/neu status in breast cancer using fluorescence in situ 
      hybridization & immunohistochemistry: Experience of a tertiary cancer referral 
      centre in India.
PG  - 287-94
AB  - BACKGROUND & OBJECTIVES: Determination of HER2 status in breast cancer has become 
      important to identify potential candidates for anti-HER2 therapy. In this study 
      we compared fluorescence in situ hybridization (FISH) and immunohistochemistry 
      (IHC) for the determination of HER2 status in breast cancer patients referred to 
      a tertiary care referral centre. METHODS: A total of 200 cases of invasive breast 
      cancer were evaluated for HER2 status using IHC and FISH and results were 
      compared. RESULTS: The IHC 3+ (93.9%) and IHC negative (85.9%) cases showed good 
      concordance with the corresponding FISH results; while 66.6 per cent of IHC 2+ 
      cases showed gene amplification by FISH. In addition, hormone receptor expression 
      and HER2 gene status showed a statistically significant inverse association 
      (P<0.05). INTERPRETATION & CONCLUSION: These findings reaffirm IHC as a prudent 
      first-step to screen tissue samples for HER2 status and to determine suitability 
      for technically demanding FISH test and the dual coloured FISH as a gold standard 
      for determination of HER2/neu status in IHC equivocal cases of breast carcinoma.
FAU - Panjwani, Poonam
AU  - Panjwani P
AD  - Department of Pathology, Tata Memorial Hospital, Dr E. Borges Road, Mumbai, 
      India.
FAU - Epari, Sridhar
AU  - Epari S
FAU - Karpate, Arti
AU  - Karpate A
FAU - Shirsat, Hemlata
AU  - Shirsat H
FAU - Rajsekharan, Preetha
AU  - Rajsekharan P
FAU - Basak, Ranjan
AU  - Basak R
FAU - Shet, Tanuja
AU  - Shet T
FAU - Chinoy, Roshni
AU  - Chinoy R
FAU - Chacko, Roy
AU  - Chacko R
FAU - Gursale, Sampada
AU  - Gursale S
FAU - Baraskar, Nayana
AU  - Baraskar N
FAU - Gupta, Sudeep
AU  - Gupta S
FAU - Hawaldar, Rohini
AU  - Hawaldar R
FAU - Desai, Sangeeta
AU  - Desai S
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - Indian J Med Res
JT  - The Indian journal of medical research
JID - 0374701
RN  - 0 (Biomarkers)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers/*analysis
MH  - Breast Neoplasms/*diagnosis/pathology
MH  - Female
MH  - Humans
MH  - Immunoenzyme Techniques/*methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - India
MH  - Male
MH  - Middle Aged
MH  - Receptor, ErbB-2/*analysis/genetics
MH  - Sensitivity and Specificity
EDAT- 2010/09/18 06:00
MHDA- 2011/01/25 06:00
CRDT- 2010/09/18 06:00
PHST- 2010/09/18 06:00 [entrez]
PHST- 2010/09/18 06:00 [pubmed]
PHST- 2011/01/25 06:00 [medline]
PST - ppublish
SO  - Indian J Med Res. 2010 Sep;132:287-94.