PMID- 20847715
OWN - NLM
STAT- MEDLINE
DCOM- 20110412
LR  - 20191210
IS  - 1534-6080 (Electronic)
IS  - 0041-1337 (Linking)
VI  - 90
IP  - 10
DP  - 2010 Nov 27
TI  - The complexity of human leukocyte antigen (HLA)-DQ antibodies and its effect on 
      virtual crossmatching.
PG  - 1117-24
LID - 10.1097/TP.0b013e3181f89c6d [doi]
AB  - BACKGROUND: We previously reported that in patients possessing human leukocyte 
      antigen (HLA)-DQ-directed antibodies, the target molecule may include the 
      patient's own DQbeta chain if it is paired with non-self-DQalpha chain, thus forming a 
      different DQ target. Herein, we sought to assess the breadth of this phenomenon. 
      METHODS: Serum samples from 104 patients awaiting kidney transplantation, known 
      to have DQ antibodies, were studied. Antibody identification was performed using 
      luminex-based HLA class II single-antigen bead assays from two vendors; DQA1/DQB1 
      typing was performed using luminex polymerase chain reaction - sequence specific 
      oligo prob hybridization (PCR-SSO) technology. RESULTS: A total of 71% of the 104 
      serum samples studied contained antibodies reactive against test beads coated 
      with the patient's own DQalpha- or beta-chain components. Of those, 35 patients (34%) 
      exhibited antibodies to their own DQbeta chain when in combination with non-self-DQalpha 
      chains; and 64 patients (62%) had antibodies to their own DQalpha chain when in 
      combination with non-self-DQbeta chains. This is a striking observation. 
      CONCLUSIONS: To the best of our knowledge, this is the first systematic, 
      high-resolution evaluation of DQ antibody repertoire. With the expansion of 
      virtual crossmatching, particularly in the context of a national registry, the 
      need for more detailed DQ antibody or antigen evaluation is critical to improve 
      operational efficiency and patient outcomes.
FAU - Tambur, Anat R
AU  - Tambur AR
AD  - Transplant Immunology Laboratory, Comprehensive Transplant Center, Feinberg 
      School of Medicine, Northwestern University, Chicago, IL, USA. 
      a-tambur@northwestern.edu
FAU - Leventhal, Joseph R
AU  - Leventhal JR
FAU - Friedewald, John J
AU  - Friedewald JJ
FAU - Ramon, Daniel S
AU  - Ramon DS
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (Autoantibodies)
RN  - 0 (Autoantigens)
RN  - 0 (Epitopes)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DQ alpha-Chains)
RN  - 0 (HLA-DQ beta-Chains)
RN  - 0 (HLA-DQA1 antigen)
RN  - 0 (HLA-DQbeta antigen)
RN  - 0 (Isoantibodies)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Alleles
MH  - Autoantibodies/immunology
MH  - Autoantigens/chemistry/genetics/immunology
MH  - Epitopes/chemistry/genetics/immunology
MH  - Female
MH  - HLA-DQ Antigens/chemistry/genetics/*immunology
MH  - HLA-DQ alpha-Chains
MH  - HLA-DQ beta-Chains
MH  - Histocompatibility Testing
MH  - Humans
MH  - Isoantibodies/*immunology
MH  - Kidney Transplantation/immunology
MH  - Linkage Disequilibrium
MH  - Male
MH  - Middle Aged
MH  - Models, Molecular
MH  - User-Computer Interface
MH  - Young Adult
EDAT- 2010/09/18 06:00
MHDA- 2011/04/13 06:00
CRDT- 2010/09/18 06:00
PHST- 2010/09/18 06:00 [entrez]
PHST- 2010/09/18 06:00 [pubmed]
PHST- 2011/04/13 06:00 [medline]
AID - 10.1097/TP.0b013e3181f89c6d [doi]
PST - ppublish
SO  - Transplantation. 2010 Nov 27;90(10):1117-24. doi: 10.1097/TP.0b013e3181f89c6d.