PMID- 20849301
OWN - NLM
STAT- MEDLINE
DCOM- 20101210
LR  - 20211020
IS  - 1931-8405 (Electronic)
IS  - 0889-2229 (Print)
IS  - 0889-2229 (Linking)
VI  - 26
IP  - 10
DP  - 2010 Oct
TI  - Analysis of selection pressure and mutational pattern of HIV type 1 reverse 
      transcriptase region among treated and nontreated patients.
PG  - 1093-6
LID - 10.1089/aid.2009.0300 [doi]
AB  - Variation of the HIV-1 subtype C reverse transcriptase region (RT) resulting in 
      response to the selective pressures of drug therapy remains poorly characterized. 
      Here, we compared the genetic variation resulting in the presence and absence of 
      antiretroviral drug selective pressures on HIV-1 subtype C RT among nontreated 
      and treated patients. The nucleotide variability, nonsynonymous and synonymous 
      ratio, and the positively selected mutations were determined by comparing the RT 
      sequences isolated at two time points among nontreated (baseline and follow-up) 
      and treated patients (baseline and treatment failure). Compared to the nontreated 
      patients, the intrapatient nucleotide variability, the number of nonsynonymous 
      and synonymous substitutions was significantly higher among the treated patients. 
      Among the mutations positively selected, the frequency of D121Y, I135R, and Q207E 
      increased and the frequency of mutation S48T decreased significantly during 
      treatment failure. Further studies are essential to discover the role of these 
      mutations during treatment in HIV-1 subtype C.
FAU - Shanmugasundaram, Uma
AU  - Shanmugasundaram U
AD  - YRG Centre for AIDS Research and Education (YRG CARE), Voluntary Health Services 
      Hospital Campus, Taramani, Chennai, India.
FAU - Solomon, Suniti
AU  - Solomon S
FAU - Madhavan, Vidya
AU  - Madhavan V
FAU - Kailapuri, Murugavel G
AU  - Kailapuri MG
FAU - Nagalingeswaran, Kumarasamy
AU  - Nagalingeswaran K
FAU - Solomon, Sunil Suhas
AU  - Solomon SS
FAU - Mayer, Kenneth H
AU  - Mayer KH
FAU - Pachamuthu, Balakrishnan
AU  - Pachamuthu B
LA  - eng
GR  - D43 TW000237/TW/FIC NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
DEP - 20100919
PL  - United States
TA  - AIDS Res Hum Retroviruses
JT  - AIDS research and human retroviruses
JID - 8709376
RN  - 0 (RNA, Viral)
RN  - 0 (Reverse Transcriptase Inhibitors)
RN  - EC 2.7.7.- (reverse transcriptase, Human immunodeficiency virus 1)
RN  - EC 2.7.7.49 (HIV Reverse Transcriptase)
SB  - IM
MH  - Adult
MH  - Drug Resistance, Multiple, Viral
MH  - Female
MH  - HIV Infections/drug therapy/genetics
MH  - HIV Reverse Transcriptase/*genetics
MH  - HIV-1/drug effects/*genetics
MH  - Humans
MH  - Male
MH  - *Mutation
MH  - RNA, Viral
MH  - Reverse Transcriptase Inhibitors/*therapeutic use
MH  - *Selection, Genetic
PMC - PMC5206687
COIS- Author Disclosure Statement No competing financial interests exist.
EDAT- 2010/09/21 06:00
MHDA- 2010/12/14 06:00
PMCR- 2011/10/01
CRDT- 2010/09/21 06:00
PHST- 2010/09/21 06:00 [entrez]
PHST- 2010/09/21 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
PHST- 2011/10/01 00:00 [pmc-release]
AID - 10.1089/aid.2009.0300 [pii]
AID - 10.1089/aid.2009.0300 [doi]
PST - ppublish
SO  - AIDS Res Hum Retroviruses. 2010 Oct;26(10):1093-6. doi: 10.1089/aid.2009.0300. 
      Epub 2010 Sep 19.