PMID- 20849589
OWN - NLM
STAT- MEDLINE
DCOM- 20101228
LR  - 20240117
IS  - 1532-429X (Electronic)
IS  - 1097-6647 (Print)
IS  - 1097-6647 (Linking)
VI  - 12
IP  - 1
DP  - 2010 Sep 17
TI  - An isolated perfused pig heart model for the development, validation and 
      translation of novel cardiovascular magnetic resonance techniques.
PG  - 53
LID - 10.1186/1532-429X-12-53 [doi]
AB  - BACKGROUND: Novel cardiovascular magnetic resonance (CMR) techniques and imaging 
      biomarkers are often validated in small animal models or empirically in patients. 
      Direct translation of small animal CMR protocols to humans is rarely possible, 
      while validation in humans is often difficult, slow and occasionally not possible 
      due to ethical considerations. The aim of this study is to overcome these 
      limitations by introducing an MR-compatible, free beating, blood-perfused, 
      isolated pig heart model for the development of novel CMR methodology. METHODS: 6 
      hearts were perfused outside of the MR environment to establish preparation 
      stability. Coronary perfusion pressure (CPP), coronary blood flow (CBF), left 
      ventricular pressure (LVP), arterial blood gas and electrolyte composition were 
      monitored over 4 hours. Further hearts were perfused within 3T (n = 3) and 1.5T 
      (n = 3) clinical MR scanners, and characterised using functional (CINE), 
      perfusion and late gadolinium enhancement (LGE) imaging. Perfusion imaging was 
      performed globally and selectively for the right (RCA) and left coronary artery 
      (LCA). In one heart the RCA perfusion territory was determined and compared to 
      infarct size after coronary occlusion. RESULTS: All physiological parameters 
      measured remained stable and within normal ranges. The model proved amenable to 
      CMR at both field strengths using typical clinical acquisitions. There was good 
      agreement between the RCA perfusion territory measured by selective first pass 
      perfusion and LGE after coronary occlusion (37% versus 36% of the LV 
      respectively). CONCLUSIONS: This flexible model allows imaging of cardiac 
      function in a controllable, beating, human-sized heart using clinical MR systems. 
      It should aid further development, validation and clinical translation of novel 
      CMR methodologies, and imaging sequences.
FAU - Schuster, Andreas
AU  - Schuster A
AD  - King's College London BHF Centre of Excellence, NIHR Biomedical Research Centre, 
      Guy's and St, Thomas' NHS Foundation Trust, Division of Imaging Sciences, The 
      Rayne Institute, London, UK.
FAU - Grunwald, Inga
AU  - Grunwald I
FAU - Chiribiri, Amedeo
AU  - Chiribiri A
FAU - Southworth, Richard
AU  - Southworth R
FAU - Ishida, Masaki
AU  - Ishida M
FAU - Hay, Gunnar
AU  - Hay G
FAU - Neumann, Nicole
AU  - Neumann N
FAU - Morton, Geraint
AU  - Morton G
FAU - Perera, Divaka
AU  - Perera D
FAU - Schaeffter, Tobias
AU  - Schaeffter T
FAU - Nagel, Eike
AU  - Nagel E
LA  - eng
GR  - FS/10/029/28253/BHF_/British Heart Foundation/United Kingdom
GR  - RE/08/003/BHF_/British Heart Foundation/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20100917
PL  - England
TA  - J Cardiovasc Magn Reson
JT  - Journal of cardiovascular magnetic resonance : official journal of the Society 
      for Cardiovascular Magnetic Resonance
JID - 9815616
RN  - 0 (Contrast Media)
RN  - 0 (Organometallic Compounds)
RN  - 1BJ477IO2L (gadobutrol)
SB  - IM
MH  - Animals
MH  - Contrast Media
MH  - *Coronary Circulation
MH  - Coronary Occlusion/complications/*diagnosis/physiopathology
MH  - Disease Models, Animal
MH  - Feasibility Studies
MH  - *Magnetic Resonance Imaging, Cine
MH  - Myocardial Infarction/*diagnosis/etiology/physiopathology
MH  - Myocardial Perfusion Imaging/*methods
MH  - Myocardium/*pathology
MH  - Organometallic Compounds
MH  - Perfusion
MH  - Predictive Value of Tests
MH  - Reproducibility of Results
MH  - Swine
MH  - *Translational Research, Biomedical
MH  - Ventricular Function
MH  - Ventricular Pressure
PMC - PMC2950014
EDAT- 2010/09/21 06:00
MHDA- 2010/12/29 06:00
PMCR- 2010/09/17
CRDT- 2010/09/21 06:00
PHST- 2010/06/21 00:00 [received]
PHST- 2010/09/17 00:00 [accepted]
PHST- 2010/09/21 06:00 [entrez]
PHST- 2010/09/21 06:00 [pubmed]
PHST- 2010/12/29 06:00 [medline]
PHST- 2010/09/17 00:00 [pmc-release]
AID - S1097-6647(23)01333-9 [pii]
AID - 1532-429X-12-53 [pii]
AID - 10.1186/1532-429X-12-53 [doi]
PST - epublish
SO  - J Cardiovasc Magn Reson. 2010 Sep 17;12(1):53. doi: 10.1186/1532-429X-12-53.