PMID- 20849897
OWN - NLM
STAT- MEDLINE
DCOM- 20110210
LR  - 20171116
IS  - 1873-5169 (Electronic)
IS  - 0196-9781 (Linking)
VI  - 31
IP  - 12
DP  - 2010 Dec
TI  - Intracerebroventricular administration of apelin-13 inhibits distal colonic 
      transit in mice.
PG  - 2241-6
LID - 10.1016/j.peptides.2010.09.006 [doi]
AB  - Apelin is a novel bioactive peptide as the endogenous ligand for the orphan 
      G-protein-coupled receptor (GPCR), APJ, a receptor distributed in various tissues 
      such as the hypothalamus and the gastrointestinal tract. Recent reports showed 
      that apelin regulated many biological functions, including blood pressure, 
      neuroendocrine, drinking behavior and food intake. However, the role of apelin in 
      regulating gastrointestinal motility remains unknown. The present study aimed to 
      investigate the actions of intracerebroventricularly administered apelin-13 on 
      colonic transit as well as the actions of apelin-13 on the contraction of 
      isolated distal colon in vitro. Intracerebroventricular (i.c.v.) injection of 
      apelin-13 (0.3, 0.5, 1 and 3 mug/mouse) dose-dependently inhibited fecal pellet 
      output and bead expulsion. This effect was significantly antagonized by the APJ 
      receptor antagonist apelin-13(F13A), indicating an APJ receptor-mediated 
      mechanism. Furthermore, naloxone could also reverse the inhibitory effect of 
      apelin-13 on fecal pellet output and bead expulsion, suggesting the involvement 
      of opioid receptors in the suppressive effect of apelin-13 on distal colon 
      transit. However, apelin-13 (10(-)(8)-10(-)(6) M) did not affect distal colonic 
      contractions in vitro.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Yang, Yan-Jie
AU  - Yang YJ
AD  - Institute of Biochemistry and Molecular Biology, School of Life Science, Lanzhou 
      University, 222 Tian Shui South Road, Lanzhou 730000, PR China.
FAU - Lv, Shuang-Yu
AU  - Lv SY
FAU - Xiu, Ming-Hui
AU  - Xiu MH
FAU - Xu, Ning
AU  - Xu N
FAU - Chen, Qiang
AU  - Chen Q
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100922
PL  - United States
TA  - Peptides
JT  - Peptides
JID - 8008690
RN  - 0 (Apelin Receptors)
RN  - 0 (Aplnr protein, mouse)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (apelin-13 peptide)
SB  - IM
MH  - Animals
MH  - Apelin Receptors
MH  - Colon/*drug effects
MH  - Feces
MH  - Gastrointestinal Transit/*drug effects
MH  - Injections, Intraventricular
MH  - Intercellular Signaling Peptides and Proteins/*pharmacology
MH  - Male
MH  - Mice
MH  - Muscle Contraction/drug effects
MH  - Muscle, Smooth/drug effects
MH  - Receptors, G-Protein-Coupled/antagonists & inhibitors
EDAT- 2010/09/21 06:00
MHDA- 2011/02/11 06:00
CRDT- 2010/09/21 06:00
PHST- 2010/07/17 00:00 [received]
PHST- 2010/09/04 00:00 [revised]
PHST- 2010/09/04 00:00 [accepted]
PHST- 2010/09/21 06:00 [entrez]
PHST- 2010/09/21 06:00 [pubmed]
PHST- 2011/02/11 06:00 [medline]
AID - S0196-9781(10)00391-8 [pii]
AID - 10.1016/j.peptides.2010.09.006 [doi]
PST - ppublish
SO  - Peptides. 2010 Dec;31(12):2241-6. doi: 10.1016/j.peptides.2010.09.006. Epub 2010 
      Sep 22.