PMID- 20851151
OWN - NLM
STAT- MEDLINE
DCOM- 20110328
LR  - 20220318
IS  - 1873-1686 (Electronic)
IS  - 0167-0115 (Linking)
VI  - 166
IP  - 1-3
DP  - 2011 Jan 17
TI  - Relaxin inhibits early steps in vascular inflammation.
PG  - 76-82
LID - 10.1016/j.regpep.2010.09.001 [doi]
AB  - Increased expression of endothelial adhesion molecules, high levels of the 
      monocyte chemoattractant protein-1 (MCP-1) and enhanced VLA4 integrin/VCAM-1 and 
      CCR-2/MCP-1 interactions are initial steps in vascular inflammation. We sought to 
      determine whether relaxin, a potent vasodilatory and anti-fibrotic agent, 
      mitigates these early events compromising endothelial integrity. The effect of 
      relaxin coincubation on the TNF-alpha-stimulated expression of the adhesion molecules 
      VCAM-1, ICAM-1 and E-selectin; the MCP-1 expression by human umbilical vein 
      endothelial cells (HUVEC) and human aortic smooth muscle cells (HAoSMC); as well 
      as on direct monocyte-endothelium cell adhesion was quantified by ELISA or 
      adhesion assay. CCR-2 and PECAM expression on HUVEC and THP-1 monocytes was 
      investigated by FACS analysis. Relaxin treatment suppressed significantly 
      TNF-alpha-induced upregulation of VCAM-1 and PECAM, CCR-2, and MCP-1 levels and 
      direct monocyte adhesion to HUVEC. Our findings identify relaxin as a promising 
      inhibitory factor in early vascular inflammation. By attenuating the upregulation 
      of VCAM-1, key adhesion molecule in early vascular inflammation, and of MCP-1, a 
      chemokine pivotal to monocyte recruitment, relaxin decreased initial 
      monocyte-endothelium contact. This may be of relevance for the prevention and 
      treatment of atherosclerosis and of other pro-inflammatory states.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Brecht, Anna
AU  - Brecht A
AD  - Department of Cardiology and Angiology, Charite-- University Medicine Berlin, 
      Berlin, Germany. anna.brecht@charite.de
FAU - Bartsch, Cornelia
AU  - Bartsch C
FAU - Baumann, Gert
AU  - Baumann G
FAU - Stangl, Karl
AU  - Stangl K
FAU - Dschietzig, Thomas
AU  - Dschietzig T
LA  - eng
PT  - Journal Article
DEP - 20100917
PL  - Netherlands
TA  - Regul Pept
JT  - Regulatory peptides
JID - 8100479
RN  - 0 (Chemokine CCL2)
RN  - 0 (E-Selectin)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 143198-26-9 (Integrin alpha4)
RN  - 9002-69-1 (Relaxin)
SB  - IM
MH  - Aorta
MH  - Cell Adhesion/drug effects
MH  - Chemokine CCL2/biosynthesis
MH  - Down-Regulation
MH  - E-Selectin/biosynthesis
MH  - Endothelium, Vascular/cytology/drug effects/metabolism
MH  - Humans
MH  - Inflammation/*prevention & control
MH  - Integrin alpha4/biosynthesis
MH  - Muscle, Smooth, Vascular/cytology
MH  - Receptors, CCR2/biosynthesis
MH  - Relaxin/*pharmacology
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Umbilical Veins
MH  - Vascular Cell Adhesion Molecule-1/biosynthesis
EDAT- 2010/09/21 06:00
MHDA- 2011/03/29 06:00
CRDT- 2010/09/21 06:00
PHST- 2010/05/03 00:00 [received]
PHST- 2010/08/22 00:00 [revised]
PHST- 2010/09/13 00:00 [accepted]
PHST- 2010/09/21 06:00 [entrez]
PHST- 2010/09/21 06:00 [pubmed]
PHST- 2011/03/29 06:00 [medline]
AID - S0167-0115(10)00361-7 [pii]
AID - 10.1016/j.regpep.2010.09.001 [doi]
PST - ppublish
SO  - Regul Pept. 2011 Jan 17;166(1-3):76-82. doi: 10.1016/j.regpep.2010.09.001. Epub 
      2010 Sep 17.