PMID- 20851650
OWN - NLM
STAT- MEDLINE
DCOM- 20110222
LR  - 20240319
IS  - 1096-0856 (Electronic)
IS  - 1090-7807 (Print)
IS  - 1090-7807 (Linking)
VI  - 207
IP  - 1
DP  - 2010 Nov
TI  - Solid-state NMR paramagnetic relaxation enhancement immersion depth studies in 
      phospholipid bilayers.
PG  - 89-94
LID - 10.1016/j.jmr.2010.08.012 [doi]
AB  - A new approach for determining the membrane immersion depth of a spin-labeled 
      probe has been developed using paramagnetic relaxation enhancement (PRE) in 
      solid-state NMR spectroscopy. A DOXYL spin label was placed at different sites of 
      1-palmitoyl-2-stearoyl-sn-glycero-3-phosphocholine (PSPC) phospholipid bilayers 
      as paramagnetic moieties and the resulting enhancements of the longitudinal 
      relaxation (T(1)) times of (3)(1)P nuclei on the surface of the bilayers were measured 
      by a standard inversion recovery pulse sequence. The (3)(1)P NMR spin-lattice 
      relaxation times decrease steadily as the DOXYL spin label moves closer to the 
      surface as well as the concentration of the spin-labeled lipids increase. The 
      enhanced relaxation vs. the position and concentration of spin-labels indicate 
      that PRE induced by the DOXYL spin label are significant to determine longer 
      distances over the whole range of the membrane depths. When these data were 
      combined with estimated correlation times tau(c), the r(-)(6)-weighted, time-averaged 
      distances between the spin-labels and the (3)(1)P nuclei on the membrane surface were 
      estimated. The application of using this solid-state NMR PRE approach coupled 
      with site-directed spin labeling (SDSL) may be a powerful method for measuring 
      membrane protein immersion depth.
CI  - Published by Elsevier Inc.
FAU - Chu, Shidong
AU  - Chu S
AD  - Department of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, 
      USA.
FAU - Maltsev, Sergey
AU  - Maltsev S
FAU - Emwas, A-H
AU  - Emwas AH
FAU - Lorigan, Gary A
AU  - Lorigan GA
LA  - eng
GR  - R01 GM080542/GM/NIGMS NIH HHS/United States
GR  - GM080542/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20100824
PL  - United States
TA  - J Magn Reson
JT  - Journal of magnetic resonance (San Diego, Calif. : 1997)
JID - 9707935
RN  - 0 (Indicators and Reagents)
RN  - 0 (Lipid Bilayers)
RN  - 0 (Phospholipids)
RN  - 0 (Sphingomyelins)
RN  - 0 (Spin Labels)
RN  - 0 (palmitoylsphingomyelin)
SB  - IM
MH  - Electron Spin Resonance Spectroscopy
MH  - Indicators and Reagents
MH  - Lipid Bilayers/*chemistry
MH  - Magnetic Resonance Spectroscopy
MH  - Phospholipids/*chemistry
MH  - Sphingomyelins
MH  - Spin Labels
PMC - PMC2978330
MID - NIHMS237950
EDAT- 2010/09/21 06:00
MHDA- 2011/02/23 06:00
PMCR- 2011/11/01
CRDT- 2010/09/21 06:00
PHST- 2010/05/26 00:00 [received]
PHST- 2010/08/11 00:00 [revised]
PHST- 2010/08/18 00:00 [accepted]
PHST- 2010/09/21 06:00 [entrez]
PHST- 2010/09/21 06:00 [pubmed]
PHST- 2011/02/23 06:00 [medline]
PHST- 2011/11/01 00:00 [pmc-release]
AID - S1090-7807(10)00252-1 [pii]
AID - 10.1016/j.jmr.2010.08.012 [doi]
PST - ppublish
SO  - J Magn Reson. 2010 Nov;207(1):89-94. doi: 10.1016/j.jmr.2010.08.012. Epub 2010 
      Aug 24.