PMID- 20854104
OWN - NLM
STAT- MEDLINE
DCOM- 20101101
LR  - 20101004
IS  - 1362-3095 (Electronic)
IS  - 0955-3002 (Linking)
VI  - 86
IP  - 10
DP  - 2010 Oct
TI  - Altered mitochondrial function and genome frequency post exposure to 
      γ-radiation and bystander factors.
PG  - 829-41
LID - 10.3109/09553002.2010.486019 [doi]
AB  - PURPOSE: To further evaluate irregular mitochondrial function and mitochondrial 
      genome damage induced by direct gamma-irradiation and bystander factors in human 
      keratinocyte (HPV-G) epithelial cells and hamster ovarian fibroblast (CHO-K1) 
      cells. This is as a follow-up to our recent reports of gamma-irradiation-induced loss 
      of mitochondrial function and mitochondrial DNA (mtDNA) damage. MATERIALS AND 
      METHODS: Mitochondrial function was evaluated post direct radiation and 
      irradiated cell conditioned medium (ICCM) by determining: Activity of the 
      individual complexes of oxidative phosphorylation (OxPhos); mtDNA-encoded protein 
      synthesis; and mitochondrial genome frequency and mtDNA damage. RESULTS: 
      Mitochondria show a loss of OxPhos enzyme function as early as 4 h post treatment 
      with recovery observed 12-96 h in some but not all complexes demonstrating a 
      non-uniform sensitivity to gamma-radiation. We also identified irregular 
      mtDNA-directed protein synthesis. Long range Polymerase Chain Reaction (PCR) 
      analysis identified mitochondrial genome damage and real-time PCR identified 
      increases in mitochondrial genome frequency. CONCLUSIONS: The study reaffirms the 
      sensitive nature of mitochondria to both low-level direct radiation exposure and 
      radiation-induced bystander factor mediated damage. Furthermore, we report for 
      the first time, the loss of function in the enzymes of OxPhos post exposure to 
      bystander factors and identify altered mtDNA-directed protein synthesis post both 
      direct radiation and bystander factors.
FAU - Nugent, Sharon
AU  - Nugent S
AD  - Radiation and Environmental Science Centre, Focas Institute, Dublin Institute of 
      Technology, Ireland.
FAU - Mothersill, Carmel E
AU  - Mothersill CE
FAU - Seymour, Colin
AU  - Seymour C
FAU - McClean, Brendan
AU  - McClean B
FAU - Lyng, Fiona M
AU  - Lyng FM
FAU - Murphy, James E J
AU  - Murphy JE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
RN  - 0 (DNA, Mitochondrial)
SB  - IM
MH  - Animals
MH  - Bystander Effect/genetics/*radiation effects
MH  - CHO Cells
MH  - Cell Line
MH  - Cricetinae
MH  - Cricetulus
MH  - DNA, Mitochondrial/genetics
MH  - Epithelial Cells/cytology/metabolism/radiation effects
MH  - *Gamma Rays
MH  - Genome/*genetics
MH  - Humans
MH  - Kinetics
MH  - Mitochondria/enzymology/genetics/*metabolism/*radiation effects
MH  - Oxidative Phosphorylation/radiation effects
MH  - Polymerase Chain Reaction
MH  - Protein Biosynthesis/radiation effects
EDAT- 2010/09/22 06:00
MHDA- 2010/11/03 06:00
CRDT- 2010/09/22 06:00
PHST- 2010/09/22 06:00 [entrez]
PHST- 2010/09/22 06:00 [pubmed]
PHST- 2010/11/03 06:00 [medline]
AID - 10.3109/09553002.2010.486019 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 2010 Oct;86(10):829-41. doi: 10.3109/09553002.2010.486019.