PMID- 20854474
OWN - NLM
STAT- MEDLINE
DCOM- 20101021
LR  - 20100921
IS  - 1600-0463 (Electronic)
IS  - 0903-4641 (Linking)
VI  - 118
IP  - 10
DP  - 2010 Oct
TI  - Increased expression of epidermal fatty acid-binding protein by alveolar 
      macrophages during acute rejection of rat lungs.
PG  - 791-800
LID - 10.1111/j.1600-0463.2010.02662.x [doi]
AB  - In the lung, epidermal fatty acid-binding protein (E-FABP) is expressed by 
      alveolar macrophages (AM) and alveolar epithelial cells type II (AEII). E-FABP 
      may regulate macrophage activation and is involved in the metabolism of 
      surfactant phospholipids. As macrophage activation and surfactant dysfunction are 
      associated with rejection, we hypothesize that E-FABP expression is changed 
      during acute rejection of pulmonary grafts. Orthotopic left lung transplantations 
      were performed in the Dark Agouti to Lewis and in the isogeneic Lewis to Lewis 
      rat strain combinations. E-FABP expression was analyzed in the lung by 
      immunohistochemistry, immunoblotting and quantitative reverse 
      transcription-polymerase chain reaction (RT-PCR). Alveolar leukocytes obtained by 
      bronchoalveolar lavage were analyzed by RT-PCR. Immunohistochemistry of isografts 
      revealed strong E-FABP immunoreactivity in AEII and a moderate immunoreactivity 
      in AM. In allografts undergoing acute rejection, AM exhibiting increased E-FABP 
      immunoreactivity accumulated. Immunoblots revealed a single band at 15 kDa, which 
      corresponds to the expected molecular mass of E-FABP. The levels of E-FABP mRNA 
      were higher in allografts than in isografts and control lungs. Furthermore, 
      alveolar leukocytes isolated by bronchoalveolar lavage from allografts displayed 
      higher E-FABP mRNA expression levels than leukocytes from isografts and controls. 
      In conclusion, we demonstrate for the first time upregulation of E-FABP 
      expression in AM during severe inflammation.
CI  - (c) 2010 The Authors. Journal Compilation (c) 2010 APMIS.
FAU - Holler, Julia
AU  - Holler J
AD  - Laboratory of Experimental Surgery, Department of General and Thoracic Surgery, 
      University of Giessen Lung Center, Justus-Liebig-University Giessen, Giessen, 
      Germany.
FAU - Zakrzewicz, Anna
AU  - Zakrzewicz A
FAU - Garn, Holger
AU  - Garn H
FAU - Hirschburger, Markus
AU  - Hirschburger M
FAU - Kummer, Wolfgang
AU  - Kummer W
FAU - Padberg, Winfried
AU  - Padberg W
FAU - Grau, Veronika
AU  - Grau V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100817
PL  - Denmark
TA  - APMIS
JT  - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
JID - 8803400
RN  - 0 (Eye Proteins)
RN  - 0 (Fabp5 protein, rat)
RN  - 0 (Fatty Acid-Binding Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Pulmonary Surfactants)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - Bronchoalveolar Lavage Fluid/cytology/immunology
MH  - Eye Proteins/*biosynthesis/genetics/immunology
MH  - Fatty Acid-Binding Proteins/*biosynthesis/genetics/immunology
MH  - Graft Rejection/*immunology
MH  - Immunity, Innate/immunology
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Lung Transplantation/*immunology
MH  - Macrophage Activation/*immunology
MH  - Macrophages, Alveolar/*immunology
MH  - Male
MH  - Nerve Tissue Proteins/*biosynthesis/genetics/immunology
MH  - Pulmonary Surfactants/immunology
MH  - RNA/chemistry/genetics
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Statistics, Nonparametric
EDAT- 2010/09/22 06:00
MHDA- 2010/10/22 06:00
CRDT- 2010/09/22 06:00
PHST- 2010/09/22 06:00 [entrez]
PHST- 2010/09/22 06:00 [pubmed]
PHST- 2010/10/22 06:00 [medline]
AID - 10.1111/j.1600-0463.2010.02662.x [doi]
PST - ppublish
SO  - APMIS. 2010 Oct;118(10):791-800. doi: 10.1111/j.1600-0463.2010.02662.x. Epub 2010 
      Aug 17.