PMID- 20854882
OWN - NLM
STAT- MEDLINE
DCOM- 20110217
LR  - 20211020
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 171
IP  - 2
DP  - 2010 Dec 1
TI  - Age-related synapse loss in hippocampal CA3 is not reversed by caloric 
      restriction.
PG  - 373-82
LID - 10.1016/j.neuroscience.2010.09.022 [doi]
AB  - Caloric restriction (CR) is a reduction of total caloric intake without a 
      decrease in micronutrients or a disproportionate reduction of any one dietary 
      component. While CR attenuates age-related cognitive deficits in tasks of 
      hippocampal-dependent memory, the cellular mechanisms by which CR improves this 
      cognitive decline are poorly understood. Previously, we have reported age-related 
      decreases in key synaptic proteins in the CA3 region of the hippocampus that are 
      stabilized by lifelong CR. In the present study, we examined possible age-related 
      changes in the functional microcircuitry of the synapses in the stratum 
      lacunosum-molecular (SL-M) of the CA3 region of the hippocampus, and whether 
      lifelong CR might prevent these age-related alterations. We used serial electron 
      microscopy to reconstruct and classify SL-M synapses and their postsynaptic 
      spines. We analyzed synapse number and size as well as spine surface area and 
      volume in young (10 months) and old (29 months) ad libitum fed rats and in old 
      rats that were calorically restricted from 4 months of age. We limited our 
      analysis to SL-M because previous work demonstrated age-related decreases in 
      synaptophysin confined to this specific layer and region of the hippocampus. The 
      results revealed an age-related decrease in macular axo-spinous synapses that was 
      not reversed by CR that occurred in the absence of changes in the size of 
      synapses or spines. Thus, the benefits of CR for CA3 function and synaptic 
      plasticity may involve other biological effects including the stabilization of 
      synaptic proteins levels in the face of age-related synapse loss.
CI  - Copyright (c) 2010 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Adams, M M
AU  - Adams MM
AD  - Department of Neurobiology, Wake Forest University School of Medicine, Medical 
      Center Boulevard, Winston-Salem, NC 27157-1010, USA. michelle@bilkent.edu.tr
FAU - Donohue, H S
AU  - Donohue HS
FAU - Linville, M C
AU  - Linville MC
FAU - Iversen, E A
AU  - Iversen EA
FAU - Newton, I G
AU  - Newton IG
FAU - Brunso-Bechtold, J K
AU  - Brunso-Bechtold JK
LA  - eng
GR  - K01 AG027828/AG/NIA NIH HHS/United States
GR  - R01 AG019886/AG/NIA NIH HHS/United States
GR  - R01 AG019886-01/AG/NIA NIH HHS/United States
GR  - R01 AG019886-04/AG/NIA NIH HHS/United States
GR  - R01 AG019886-02/AG/NIA NIH HHS/United States
GR  - K01 AG027828-02/AG/NIA NIH HHS/United States
GR  - K01 AG027828-03/AG/NIA NIH HHS/United States
GR  - K01 AG027828-01/AG/NIA NIH HHS/United States
GR  - P01 AG011370-110003/AG/NIA NIH HHS/United States
GR  - P01 AG011370-16/AG/NIA NIH HHS/United States
GR  - R01 AG019886-05/AG/NIA NIH HHS/United States
GR  - P01 AG011370-150003/AG/NIA NIH HHS/United States
GR  - P01 AG11370/AG/NIA NIH HHS/United States
GR  - P01 AG011370-130003/AG/NIA NIH HHS/United States
GR  - R01 AG019886-03/AG/NIA NIH HHS/United States
GR  - P01 AG011370/AG/NIA NIH HHS/United States
GR  - P01 AG011370-120003/AG/NIA NIH HHS/United States
GR  - P01 AG011370-140003/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20100918
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Synaptophysin)
SB  - IM
MH  - Aging/*pathology
MH  - Animals
MH  - CA3 Region, Hippocampal/metabolism/*ultrastructure
MH  - *Caloric Restriction
MH  - Dendritic Spines/metabolism/ultrastructure
MH  - Male
MH  - Rats
MH  - Rats, Inbred F344
MH  - Synapses/metabolism/*ultrastructure
MH  - Synaptophysin/metabolism
PMC - PMC2995889
MID - NIHMS238985
EDAT- 2010/09/22 06:00
MHDA- 2011/02/18 06:00
PMCR- 2011/12/01
CRDT- 2010/09/22 06:00
PHST- 2010/08/06 00:00 [received]
PHST- 2010/09/01 00:00 [revised]
PHST- 2010/09/14 00:00 [accepted]
PHST- 2010/09/22 06:00 [entrez]
PHST- 2010/09/22 06:00 [pubmed]
PHST- 2011/02/18 06:00 [medline]
PHST- 2011/12/01 00:00 [pmc-release]
AID - S0306-4522(10)01269-8 [pii]
AID - 10.1016/j.neuroscience.2010.09.022 [doi]
PST - ppublish
SO  - Neuroscience. 2010 Dec 1;171(2):373-82. doi: 10.1016/j.neuroscience.2010.09.022. 
      Epub 2010 Sep 18.