PMID- 20855644
OWN - NLM
STAT- MEDLINE
DCOM- 20101018
LR  - 20151119
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 134
IP  - 4
DP  - 2010 Oct
TI  - The reliability of rabbit monoclonal antibodies in the immunohistochemical 
      assessment of estrogen receptors, progesterone receptors, and HER2 in human 
      breast carcinomas.
PG  - 621-32
LID - 10.1309/AJCPOG3O3KTPZQNK [doi]
AB  - The reliability of the rabbit monoclonal antibodies SP1, SP2, SP3, and 4B5 was 
      immunohistochemically assessed on a range of 96 invasive breast carcinomas and 
      the results compared with those achieved with established antibody markers for 
      estrogen receptors (6F11), progesterone receptors (PgR636), and HER2 (polyclonal 
      A0485 and clone CB11), with HER2 status validated by fluorescence in situ 
      hybridization (FISH) and silver in situ hybridization. Optimal results depended 
      on the duration of microwave antigen-retrieval time and the use of a high pH 
      buffer for rabbit and mouse estrogen receptor antibodies (SP1 and 6F11), although 
      only on antigen-retrieval duration for the progesterone receptors SP2 and PgR636. 
      The highest rate of concordance between HER2 overexpression and HER2 gene 
      amplification was with the rabbit monoclonal antibodies (SP3 and 4B5) and FISH. 
      Rabbit monoclonal antibodies are reliable alternatives to established antibody 
      markers for the immunohistochemical testing of estrogen receptors, progesterone 
      receptors, and HER2 in breast cancer.
FAU - Rhodes, Anthony
AU  - Rhodes A
AD  - Faculty of Health & Life Sciences, University of the West of England, Coldharbour 
      Lane, Bristol, BS16 1QY, England.
FAU - Sarson, Julia
AU  - Sarson J
FAU - Assam, Emma E
AU  - Assam EE
FAU - Dean, Sarah J R
AU  - Dean SJ
FAU - Cribb, Edward C
AU  - Cribb EC
FAU - Parker, Andrew
AU  - Parker A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Receptors, Progesterone)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Animals
MH  - *Antibodies, Monoclonal
MH  - Biomarkers, Tumor/*analysis
MH  - Breast Neoplasms/*chemistry/pathology
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Microarray Analysis/methods
MH  - Rabbits
MH  - Receptor, ErbB-2/*analysis
MH  - Receptors, Estrogen/*analysis
MH  - Receptors, Progesterone/*analysis
EDAT- 2010/09/22 06:00
MHDA- 2010/10/19 06:00
CRDT- 2010/09/22 06:00
PHST- 2010/09/22 06:00 [entrez]
PHST- 2010/09/22 06:00 [pubmed]
PHST- 2010/10/19 06:00 [medline]
AID - 134/4/621 [pii]
AID - 10.1309/AJCPOG3O3KTPZQNK [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2010 Oct;134(4):621-32. doi: 10.1309/AJCPOG3O3KTPZQNK.