PMID- 20855890
OWN - NLM
STAT- MEDLINE
DCOM- 20101230
LR  - 20211020
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 285
IP  - 48
DP  - 2010 Nov 26
TI  - HNK-1 epitope-carrying tenascin-C spliced variant regulates the proliferation of 
      mouse embryonic neural stem cells.
PG  - 37293-301
LID - 10.1074/jbc.M110.157081 [doi]
AB  - Neural stem cells (NSCs) possess high proliferative potential and the capacity 
      for self-renewal with retention of multipotency to differentiate into neuronal 
      and glial cells. NSCs are the source for neurogenesis during central nervous 
      system development from fetal and adult stages. Although the human natural 
      killer-1 (HNK-1) carbohydrate epitope is expressed predominantly in the nervous 
      system and involved in intercellular adhesion, cell migration, and synaptic 
      plasticity, the expression patterns and functional roles of HNK-1-containing 
      glycoconjugates in NSCs have not been fully recognized. We found that HNK-1 was 
      expressed in embryonic mouse NSCs and that this expression was lost during the 
      process of differentiation. Based on proteomics analysis, it was revealed that 
      the HNK-1 epitopes were almost exclusively displayed on an extracellular matrix 
      protein, tenascin-C (TNC), in the mouse embryonic NSCs. Furthermore, the HNK-1 
      epitope was found to be present only on the largest isoform of the TNC molecules. 
      In addition, the expression of HNK-1 was dependent on expression of the largest 
      TNC variant but not by enzymes involved in the biosynthesis of HNK-1. By knocking 
      down HNK-1 sulfotransferase or TNC by small interfering RNA, we further 
      demonstrated that HNK-1 on TNC was involved in the proliferation of NSCs via 
      modulation of the expression level of the epidermal growth factor receptor. Our 
      finding provides insights into the function of HNK-1 carbohydrate epitopes in 
      NSCs to maintain stemness during neural development.
FAU - Yagi, Hirokazu
AU  - Yagi H
AD  - From the Institute of Molecular Medicine and Genetics and Institute of 
      Neuroscience, Medical College of Georgia, Augusta, Georgia 30912.
FAU - Yanagisawa, Makoto
AU  - Yanagisawa M
FAU - Suzuki, Yusuke
AU  - Suzuki Y
FAU - Nakatani, Yoshihiko
AU  - Nakatani Y
FAU - Ariga, Toshio
AU  - Ariga T
FAU - Kato, Koichi
AU  - Kato K
FAU - Yu, Robert K
AU  - Yu RK
LA  - eng
GR  - R01 NS011853/NS/NINDS NIH HHS/United States
GR  - R01 NS026994/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100920
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (CD57 Antigens)
RN  - 0 (Epitopes)
RN  - 0 (Tenascin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - CD57 Antigens/chemistry/genetics/*metabolism
MH  - Cell Differentiation
MH  - *Cell Proliferation
MH  - Cells, Cultured
MH  - Epitopes/chemistry/genetics/*metabolism
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Mice, Knockout
MH  - Molecular Sequence Data
MH  - Neural Stem Cells/chemistry/*cytology/metabolism
MH  - Protein Structure, Tertiary
MH  - *RNA Splicing
MH  - Sequence Alignment
MH  - Tenascin/*chemistry/genetics/*metabolism
PMC - PMC2988335
EDAT- 2010/09/22 06:00
MHDA- 2010/12/31 06:00
PMCR- 2011/11/26
CRDT- 2010/09/22 06:00
PHST- 2010/09/22 06:00 [entrez]
PHST- 2010/09/22 06:00 [pubmed]
PHST- 2010/12/31 06:00 [medline]
PHST- 2011/11/26 00:00 [pmc-release]
AID - S0021-9258(20)46705-5 [pii]
AID - M110.157081 [pii]
AID - 10.1074/jbc.M110.157081 [doi]
PST - ppublish
SO  - J Biol Chem. 2010 Nov 26;285(48):37293-301. doi: 10.1074/jbc.M110.157081. Epub 
      2010 Sep 20.