PMID- 20860556
OWN - NLM
STAT- MEDLINE
DCOM- 20110517
LR  - 20110114
IS  - 1365-3164 (Electronic)
IS  - 0959-4493 (Linking)
VI  - 22
IP  - 1
DP  - 2011 Feb
TI  - Effect of recombinant canine interferon-gamma on granulocyte-macrophage 
      colony-stimulating factor, transforming growth factor-beta and CC chemokine ligand 
      17 mRNA transcription in a canine keratinocyte cell line (CPEK).
PG  - 24-30
LID - 10.1111/j.1365-3164.2010.00897.x [doi]
AB  - Recombinant canine interferon-gamma (rCaIFN-gamma) produced by a baculovirus expression 
      system has therapeutic efficacy against atopic dermatitis in dogs. Although the 
      mechanism of action of rCaIFN-gamma is not completely understood, rCaIFN-gamma is thought 
      to downregulate the activity of interleukin-4- and interleukin-5-producing T 
      helper 2 cells. However, rCaIFN-gamma may also act directly on canine keratinocytes 
      by inhibiting the release of inflammatory mediators. In this study, we 
      investigated the effects of rCaIFN-gamma on cytokine and chemokine mRNA transcription 
      in a canine keratinocyte cell line, CPEK. It was found that 
      granulocyte-macrophage colony-stimulating factor (GM-CSF) mRNA transcription was 
      significantly inhibited after treatment with rCaIFN-gamma (P<0.001), whereas 
      transforming growth factor-beta and CC chemokine ligand 17 mRNA levels were 
      unchanged. This study suggests that rCaIFN-gamma may suppress GM-CSF production from 
      canine keratinocytes, although further studies are required to confirm this.
CI  - (c) 2010 The Authors. Journal compilation (c) 2010 ESVD and ACVD.
FAU - Shibata, Sanae
AU  - Shibata S
AD  - The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, 
      Gifu, Japan.
FAU - Maeda, Sadatoshi
AU  - Maeda S
FAU - Kondo, Naho
AU  - Kondo N
FAU - Inoue, Akiko
AU  - Inoue A
FAU - Maeda, Shingo
AU  - Maeda S
FAU - Chimura, Naoki
AU  - Chimura N
FAU - Fukata, Tsuneo
AU  - Fukata T
LA  - eng
PT  - Journal Article
DEP - 20100921
PL  - England
TA  - Vet Dermatol
JT  - Veterinary dermatology
JID - 9426187
RN  - 0 (Chemokine CCL17)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transforming Growth Factor beta)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chemokine CCL17/genetics/*metabolism
MH  - Dogs
MH  - Dose-Response Relationship, Drug
MH  - Gene Expression Regulation/drug effects
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/genetics/*metabolism
MH  - Interferon-gamma/*pharmacology
MH  - Keratinocytes/*drug effects/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Recombinant Proteins
MH  - Transforming Growth Factor beta/genetics/*metabolism
EDAT- 2010/09/24 06:00
MHDA- 2011/05/18 06:00
CRDT- 2010/09/24 06:00
PHST- 2010/09/24 06:00 [entrez]
PHST- 2010/09/24 06:00 [pubmed]
PHST- 2011/05/18 06:00 [medline]
AID - 10.1111/j.1365-3164.2010.00897.x [doi]
PST - ppublish
SO  - Vet Dermatol. 2011 Feb;22(1):24-30. doi: 10.1111/j.1365-3164.2010.00897.x. Epub 
      2010 Sep 21.