PMID- 20860616
OWN - NLM
STAT- MEDLINE
DCOM- 20110315
LR  - 20211020
IS  - 1530-0277 (Electronic)
IS  - 0145-6008 (Print)
IS  - 0145-6008 (Linking)
VI  - 34
IP  - 12
DP  - 2010 Dec
TI  - Implications of ER stress, the unfolded protein response, and pro- and 
      anti-apoptotic protein fingerprints in human monocyte-derived dendritic cells 
      treated with alcohol.
PG  - 2081-8
LID - 10.1111/j.1530-0277.2010.01304.x [doi]
AB  - BACKGROUND: Dendritic cells (DCs) are responsible for the activation of T cells 
      and B cells. There is accumulating evidence that psychoactive substances such as 
      alcohol can affect immune responses. We hypothesize that this occurs by 
      modulating changes in proteins triggering a process known as unfolded protein 
      response (UPR). This process protects cells from the toxic effects of misfolded 
      proteins responsible for causing endoplasmic reticulum (ER) stress. Although much 
      is known about ER stress, little is understood about the consequences of ethanol 
      use on DC's protein expression. METHODS: In this study, we investigated 
      alterations in the proteins of human monocyte-derived dendritic cells (MDDC) 
      treated with 0.1% of alcohol by two-dimensional (2D) gel electrophoresis followed 
      by liquid chromatography-tandem mass spectrometry, protein identification, and 
      confirmation at the gene expression level by qRT-PCR. RESULTS: Proteomes of 
      related samples demonstrated 32 differentially expressed proteins that had a 
      2-fold or greater change in expression (18 spots were up-regulated and 14 were 
      down-regulated), compared to the control cultures (untreated cells). Alcohol 
      significantly changed the expression of several components of the UPR 
      stress-induced pathways that include chaperones, ER stress, antioxidant enzymes, 
      proteases, alcohol dehydrogenase, cytoskeletal and apoptosis-regulating proteins. 
      qRT-PCR analyses highlighted the enhanced expression of UPR and antioxidant genes 
      that increased (18 hours) with alcohol treatment. CONCLUSION: Results of these 
      analyses provide insights into alcohol mechanisms of regulating DC and suggest 
      that alcohol induced specifically the UPR in DC. We speculate that activation of 
      a UPR by alcohol may protect the DC from oxidant injury but may lead to the 
      development of alcohol-related diseases.
CI  - Copyright (c) 2010 by the Research Society on Alcoholism.
FAU - Boukli, Nawal M
AU  - Boukli NM
AD  - Biomedical Proteomics Facility, Department of Microbiology and Immunology, 
      Universidad Central del Caribe School of Medicine, Bayamon, Puerto Rico, USA.
FAU - Saiyed, Zainulabedin M
AU  - Saiyed ZM
FAU - Ricaurte, Martha
AU  - Ricaurte M
FAU - Rodriguez, Jose W
AU  - Rodriguez JW
FAU - Rios Olivares, Eddy
AU  - Rios Olivares E
FAU - Cubano, Luis A
AU  - Cubano LA
FAU - Nair, Madhavan P N
AU  - Nair MP
LA  - eng
GR  - R01 MH085259/MH/NIMH NIH HHS/United States
GR  - R01 AA017405/AA/NIAAA NIH HHS/United States
GR  - R01 DA021537/DA/NIDA NIH HHS/United States
GR  - G12 MD007583/MD/NIMHD NIH HHS/United States
GR  - R01 DA012366-11/DA/NIDA NIH HHS/United States
GR  - R37DA025576/DA/NIDA NIH HHS/United States
GR  - G11 HD052352/HD/NICHD NIH HHS/United States
GR  - R01 DA027049/DA/NIDA NIH HHS/United States
GR  - S06GM050695/GM/NIGMS NIH HHS/United States
GR  - G11HD052352/HD/NICHD NIH HHS/United States
GR  - R01DA027049/DA/NIDA NIH HHS/United States
GR  - R01DA021537/DA/NIDA NIH HHS/United States
GR  - R01 DA027049-03/DA/NIDA NIH HHS/United States
GR  - 2G12RR03035/RR/NCRR NIH HHS/United States
GR  - R01AA017405/AA/NIAAA NIH HHS/United States
GR  - R01DA012366/DA/NIDA NIH HHS/United States
GR  - R01 DA012366/DA/NIDA NIH HHS/United States
GR  - R01 MH085259-02/MH/NIMH NIH HHS/United States
GR  - R37 DA025576-03/DA/NIDA NIH HHS/United States
GR  - S06 GM050695/GM/NIGMS NIH HHS/United States
GR  - R01 DA021537-06/DA/NIDA NIH HHS/United States
GR  - G12 RR003035/RR/NCRR NIH HHS/United States
GR  - R37 DA025576/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20100922
PL  - England
TA  - Alcohol Clin Exp Res
JT  - Alcoholism, clinical and experimental research
JID - 7707242
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Proteome)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Apoptosis Regulatory Proteins/*metabolism
MH  - Cells, Cultured
MH  - Dendritic Cells/drug effects/*metabolism
MH  - Endoplasmic Reticulum/drug effects/*metabolism
MH  - Ethanol/*pharmacology
MH  - Gene Expression/drug effects
MH  - Humans
MH  - Proteome/drug effects
MH  - Stress, Physiological/*drug effects
MH  - Unfolded Protein Response/*drug effects
PMC - PMC2988982
MID - NIHMS225615
EDAT- 2010/09/24 06:00
MHDA- 2011/03/16 06:00
PMCR- 2011/12/01
CRDT- 2010/09/24 06:00
PHST- 2010/09/24 06:00 [entrez]
PHST- 2010/09/24 06:00 [pubmed]
PHST- 2011/03/16 06:00 [medline]
PHST- 2011/12/01 00:00 [pmc-release]
AID - 10.1111/j.1530-0277.2010.01304.x [doi]
PST - ppublish
SO  - Alcohol Clin Exp Res. 2010 Dec;34(12):2081-8. doi: 
      10.1111/j.1530-0277.2010.01304.x. Epub 2010 Sep 22.